Summary
Median volumes ofin vitro coelomocyte populations fromGlycera dibranchiata rapidly change in response to external differences of osmotic pressure (Fig. 3). Fifty per cent haemolysis occurred in just under 30% sea water, 285 mOSm·kg−1. Hydraulic conductivities (Lp=0.92 to 2.78×107 cm×s−1·atm−1) calculated from rates of osmotic swelling were similar to values for sea urchin eggs and squid axons. Coelomocytes show a slower partial return to their original volumes in hypotonic but not hypertonic media. This asymmetry is reflected in Ponder's R values of 0.787 and 0.987, respectively, determined after this regulatory phase is complete (Figs. 4 and 5). Evidence for an irreversible stress dependent leakage of osmotically active solutes when the coelomocytes are removed from the animal and diluted with sea water is presented.
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Machin, J., O'Donnell, M.J. Volume regulation in the coelomocytes of the blood wormGlycera dibranchiata . J Comp Physiol B 117, 303–311 (1977). https://doi.org/10.1007/BF00691556
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DOI: https://doi.org/10.1007/BF00691556