Abstract
Leaf protoplasts were isolated from axenic shoot cultures of four varieties of Capsicum annuum (Americano, Dulce Italiano Florida Gynat and Nigrum) and a wild species C. chinense. Protoplasts of both species, cultured in KM8P medium and using agarose bead culture, entered division with the exception of the variety Nigrum. Cell colonies formed callus in agar-solified MS medium supplemented with zeatin and for C. annuum v. Dulce Italiano shoots were regenerated when protoplast-derived calli were transferred to MS medium with 6-BAP. Excised shoots were rooted on MS medium which lacked phytohormones.
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Abbreviations
- 6-BAP:
-
6-benzylamino purine
- MS:
-
Murashige and Skoog (1962)
- KM8P:
-
Kao and Michayluk (1975)
- SDS:
-
sodium dodecyl sulphate
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Communicated by I. Potrykus
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Díaz, I., Moreno, R. & Power, J.B. Plant regeneration from protoplasts of Capsicum annuum . Plant Cell Reports 7, 210–212 (1988). https://doi.org/10.1007/BF00269326
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DOI: https://doi.org/10.1007/BF00269326