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A simple, accurate method for determining wet and dry weight concentrations of plant cell suspension cultures using microcentrifuge tubes

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Summary

A simple method using microcentrifuge tubes for determining fresh and dry weights, and collecting cell-free supernatant from plant suspension cultures is described. This method offers improvements in accuracy, precision, and time efficiency over traditional filtration methods. Using 4-day-old Nicotinia tabacum cultures, the centrifuge method was shown to remove 25% more of the interstitial water from cell aggregates compared to a suction filtration method, with significantly less variation in fresh weight data.

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Authors and Affiliations

  1. Department of Chemical Engineering, Washington State University, 99164-2710, Pullman, WA, USA

    David R. Mills & James M. Lee

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  1. David R. Mills
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  2. James M. Lee
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Communicated by F. Constabel

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Mills, D.R., Lee, J.M. A simple, accurate method for determining wet and dry weight concentrations of plant cell suspension cultures using microcentrifuge tubes. Plant Cell Reports 15, 634–636 (1996). https://doi.org/10.1007/BF00232467

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  • DOI: https://doi.org/10.1007/BF00232467

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