Summary
A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 μm DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.
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Communicated by B.S. Cox
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Ono, Bi., Heike, C., Yano, Y. et al. Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae . Curr Genet 21, 285–289 (1992). https://doi.org/10.1007/BF00351684
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DOI: https://doi.org/10.1007/BF00351684