Summary
A spontaneous high-level cycloheximide-resistant mutant of the yeast Saccharomyces cerevisiae (strain cy32) is found to have an altered protein of the large subunit (60S) of cytoplasmic ribosomes, namely protein L29. The resistance character segregates together with this biochemical defect and is semidominant in heterozygous diploids. Judged from in vitro susceptibility to inhibition by cycloheximide there are at least 50% resistant ribosomes present in such diploid strains. From these results it is concluded that cycloheximide resistance of mutant cy32 is caused by mutation of a single gene and that it is the structural gene for L29 which is affected.
Preliminary genetic mapping data are also reported. They indicate a location of cyhx-32 marker on chromosome 7 near met13.
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Bégueret, J., Perrot, M., Crouzet, M.: Mol. Gen. Genet. 156, 141–144 (1977)
Coddington, A., Fluri, R.: Mol. Gen. Genet. 158, 93–100 (1977)
Cooper, D., Banthorpe, D., Wilkie, D.: J. Mol. Biol. 26, 347–350 (1967)
Cooper, T. G., Bossinger, J.: J. Biol. Chem. 251, 7278–7280 (1976)
Grant, P., Sanchez, L., Jiménez, A.: J. Bacteriol. 120, 1308–1314 (1974
Hardy, S. J. S., Kurland, C. G., Voynow, P., Mora, G.: Biochemistry 8, 2897–2905 (1969)
Haugli, F. B., Dove, W. F., Jiménez, A.: Mol. Gen. Genet. 118, 97–107 (1972)
Hawthorne, D. C., Mortimer, R. K.: In: Handbook of biochemistry and molecular biology (G. D. Fasman, ed.), 3rd edition, nucleic acids - vol. 2, pp. 765–832. Cleveland: CRC Press 1976
Jiménez, A., Littlewood, B., Davies, J.: In: Molecular mechanisms of antibiotic action on protein synthesis and membranes (E. Munoz, F. Garaia-Ferrandiz, D. Vazquez, eds.), pp. 292–306. New York: Elsevier 1972
Kaltschmidt, E., Wittmann, H. G.: Anal. Biochem. 36, 401–412 (1970)
Kruiswijk, T., Planta, R. J.: Mol. Biol. Rep. 1, 409–415 (1974)
Laemmli, U. K.: Nature 227, 680–685 (1970)
McLaughlin, C. S.: In: Ribosomes (M. Nomura, A. Tissières, P. Lengyel, eds.). Cold Spring Harbor Monograph Series, pp. 815–827. New York: Cold Spring Harbor Laboratory 1974
Mets, L. J., Bogorad, L.: Anal. Biochem. 57, 200–210 (1974)
Oleinick, N. L.: Arch. Biochem. Biophys. 182, 171–180 (1977)
Otaka, E., Kobata, K.: Mol. Gen. Genet. 162, 259–268 (1978)
Pestka, S.: In: Molecular mechanisms of protein biosynthesis (H. Weissbach, S. Pestka, eds.), pp. 467–553. New York, San Francisco, London: Academic Press 1977
Pöche, H., Junghahn, J., Geissler, E., Bielka, H.: Mol. Gen. Genet. 138, 173–177 (1975)
Pongratz, M., Klingmüller, W.: Mol. Gen. Genet. 124, 359–363 (1973)
Rao, S. S., Grollmann, A. P.: Biochem. Biophys. Res. Commun. 29, 696–704 (1967)
Sherman, F., Fink, G. R., Lawrence, C. W.: Methods in yeast genetics (Laboratory manual for a course). New York: Cold Spring Harbor Laboratory 1977 (revised version)
Somasundaran, U., Skogerson, L.: Biochemistry 15, 4760–4764 (1976)
Sutton, C. A., Ares, M., Hallberg, R. L.: Proc. Nat. Acad. Sci. USA 75, 3158–3162 (1978)
Vazquez, D.: In: Molecular biology, biochemistry and biophysics (A. Kleinzeller, G. F. Springer, H. G. Wittmann, eds.), Vol. 30. Berlin, Heidelberg, New York: Springer 1979
Waldron, C., Box, B. S.: Mol. Gen. Genet. 159, 223–225 (1978)
Wilkie, D., Lee, B. K.: Genet. Res. Cam. 6, 130–138 (1965)
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Some of the data delt with in this paper were reported on the IX. Steenbock Symposium on Ribosomes, July 5th to 8th, 1979, Madison (Wisc.), U.S.A.
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Stöcklein, W., Piepersberg, W. Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiae . Curr Genet 1, 177–183 (1980). https://doi.org/10.1007/BF00390941
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DOI: https://doi.org/10.1007/BF00390941