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Regulation of scytophycin accumulation in cultures of Scytonema ocellatum. II. Nutrient requirements

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Abstract

The effects of optimal sources and concentrations of major nutrients (supplying N, S, P, K+, Na+, Ca2+, Mg2+, and inorganic carbon) and organic buffers on growth and secondary metabolite accumulation in Scytonema ocellatum strain FF-66-3 were determined. Nitrate, phosphate, magnesium, and sulfur had no specific stimulatory or inhibitory effects on scytophycin accumulation within the range of concentrations that supported optimal growth. Calcium concentrations greater than those required for growth (0.1 mM) stimulated scytophycin accumulation. Sodium carbonate concentrations in excess of 0.25 mM strongly inhibited growth. Ammonium (2.5 mM) inhibited both growth and product formation. 3-[N-Morpholino]propanesulfonic acid at 3–5 mM effectively controlled pH and facilitated both growth and product formation.

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References

  • Aharonowitz Y (1980) Nitrogen regulation of antibiotic biosynthesis. Annu Rev Microbiol 34:209–233

    Google Scholar 

  • Bhattacharya M, Majumdar SK (1982) Studies on the nutritional requirements of Penicillium citrinum for the synthesis of citrinin toxin. J Food Sci Technol 19:61–63

    Google Scholar 

  • Bloor S, England RR (1991) Elucidation and optimization of the medium constituents controlling antibiotic production by the cyanobacterium Nostoc muscorum. Enzyme Microb Technol 13:76–81

    Google Scholar 

  • Carmeli S, Moore RE, Patterson GML (1990) Tolytoxin and new scytophycins from three species of Scytonema. J Nat Prod 53:1533–1542

    Google Scholar 

  • Carmichael WW (1992) Cyanobacteria secondary metabolites-the cyanotoxins. J Appl Bacteriol 72:445–459

    Google Scholar 

  • Castenholz RW (1988) Culture methods for cyanobacteria. Methods Enzymol 167:68–93

    Google Scholar 

  • Flores E, Wolk CP (1986) Production, by filamentous, nitrogen-fixing cyanobacteria, of a bacteriocin and of other antibiotics that kill related strains. Arch Microbiol 145:215–219

    Google Scholar 

  • Foerster JW (1964) The use of calcium and magnesium hardness ions to stimulate sheath formation in Oscillatoria limosa (Roth) C. A. Agardh. Trans Am Microsc Soc 83:420–427

    Google Scholar 

  • Fogg GE, Thake B (1987) Algal cultures and phytoplankton ecology, 3rd edn. University of Wisconsin Press, Madison

    Google Scholar 

  • Garner HR, Fahmy M, Phillips RL, Koffler H, Tetrault PA, Bohonos N (1950) Chemical changes in the submerged growth of Streptomyces griseus. Bacteriol Proc 1:139–140

    Google Scholar 

  • Gentile JH, Maloney TE (1969) Toxicity and environmental requirements of a strains of Aphanizomenon flos-aquae. Can J Microbiol 15:165–173

    Google Scholar 

  • Gleason FD, Wood JM (1987) Secondary metabolism in cyanobacteria. In: Fay P, Van Baalen C (eds), The cyanobacteria. Elsevier, Amsterdam, p 437

    Google Scholar 

  • Hobbs G, Frazer CM, Gardner DCJ, Flett F, Oliver SG (1990) Pigmented antibiotic production by Streptomyces coelicolor A3(2): kinetics and the influence of nutrients. J Gen Microbiol 136:2291–2296

    Google Scholar 

  • Jung JH, Moore RE, Patterson GML (1991) Scytophycins from a blue-green alga belonging to the Nostocaceae. Phytochemistry 30:3615–3616

    Google Scholar 

  • Kratz WA, Myers J (1955) Nutrition and growth of several blue-green algae. Am J Bot 42:282–287

    Google Scholar 

  • Kumar HD, Prakash G (1971) Toxicity of selenium to the blue-green algae, Anacystis nidulans and Anabaena variabilis. Ann Bot 35:697–705

    Google Scholar 

  • Majumdar MK, Majumdar SK (1965) Effects of minerals on neomycin production by Streptomyces fradiae. Appl Microbiol 13:190–193

    Google Scholar 

  • Manabe E, Hirano M, Takano H, Ishikawa-Doi N, Sode K, Matsunaga T (1992) Influence of ammonium chloride on growth and fatty acid production by Spirulina platensis. Appl Biochem Biotechnol 34:273–281

    Google Scholar 

  • Martín JF, Demain AL (1980) Control of antibiotic biosynthesis. Microbiol Rev 44:230–251

    Google Scholar 

  • Neidhardt FC, Bloch PL, Smith DF (1974) Culture medium for Enterobacteria. J Bacteriol 119:736–747

    Google Scholar 

  • Nomi R (1963) Streptomycin formation by intact mycelium of Streptomyces griseus. J Bacteriol 86:1220–1230

    Google Scholar 

  • O'Flaherty LM, Phinney HK (1970) Requirements for the maintenance and growth of Aphanizomenon flos-aquae in culture. J Phycol 6:95–97

    Google Scholar 

  • Patterson GML, Bolis C (1993a) Regulation of scytophycin accumulation in cultures of Scytonema ocellatum. I. Physical factors. Appl Microbiol Biotechnol 40:375–381

    Google Scholar 

  • Patterson GML, Bolis C (1993b) Determination of scytophycins in cyanophyte cultures by high performance liquid chromatography. J Liq Chromatogr 16:475–486

    Google Scholar 

  • Patterson GML, Baldwin CL, Bolis CM, Caplan FR, Karuso H, Larsen LK, Levine IA, Moore RE, Nelson CS, Tuang GD, Furusawa E, Furusawa S, Norton TR, Raybourne RR (1991) Antineoplastic activity of cultured blue-green algae (cyanobacteria). J Phycol 27:530–536

    Google Scholar 

  • Patterson GML, Smith CD, Kimura LH, Britton BA, Carmeli S (1993a) Action of tolytoxin on cell morphology, cytoskeletal organization, and actin polymerization. Cell Motil Cytoskeleton 24:39–48

    Google Scholar 

  • Patterson GML, Baker KK, Baldwin CL, Bolis CM, Caplan FR, Larsen LK, Levine IA, Moore RE, Nelson CS, Tschappat KD, Tuang GD, Boyd MR, Cardellina JH II, Collins RP, Gustafson KR, Snader KM, Weislow OS (1993b) Antiviral activity of cultured blue-green algae (cyanophyta). J Phycol 29:125–130

    Google Scholar 

  • Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  • Schwartz RE, Hirsch CF, Sesin DF, Flor JE, Chartrain M, Fromtling RE, Harris GH, Salvatore MJ, Liesch JM, Yudin K (1990) Pharmaceuticals from cultured algae. J Ind Microbiol 5:113–124

    Google Scholar 

  • Sivonen K (1990) Effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by Oscillatoria agardhii strains. App Environ Microbiol 56:2658–2666

    Google Scholar 

  • Smith CD, Carmeli S, Moore RE, Patterson GML (1993) Scytophycins: novel microfilament-depolymerizing agents which circumvent p-glycoprotein-mediated multidrug resistance. Cancer Res 53:1343–1347

    Google Scholar 

  • Smith RJ (1988) Calcium-mediated regulation in the cyanobacteria? In: Rogers LJ, Gallon JR (eds) Biochemistry of the Algae and Cyanobacteria. Oxford University Press, Oxford, p 185

    Google Scholar 

  • Wälzlein G, Pistorius EK (1991) Inactivation of photosynthetic O2 evolution in the cyanobacterium Anacystis nidulans PCC6301: influence of nitrogen metabolites and divalent cation concentration. z Naturforsch 46c:1024–1032

    Google Scholar 

  • Wolk CP (1973) Physiology and cytological chemistry of blue-green algae. Bacteriol Rev 37:32–101

    Google Scholar 

  • Yongmanitchai W, Ward OP (1991) Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl Environ Microbiol 57:419–425

    Google Scholar 

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  1. Department of Chemistry, University of Hawaii at Manoa, 96822, Honolulu, HI, USA

    G. M. L. Patterson & C. M. Bolis

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  1. G. M. L. Patterson
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  2. C. M. Bolis
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Patterson, G.M.L., Bolis, C.M. Regulation of scytophycin accumulation in cultures of Scytonema ocellatum. II. Nutrient requirements. Appl Microbiol Biotechnol 43, 692–700 (1995). https://doi.org/10.1007/BF00164775

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  • DOI: https://doi.org/10.1007/BF00164775

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