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Biodegradation of 2-chloroethanol by freely suspended and adsorbed immobilized Pseudomonas putida US2 in soil

  • Environmental Biotechnology
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Abstract

The degradation of 2-chloroethanol by Pseudomonas putida US2 was investigated in batch, repeated batch and continuous cultures especially in a packed-bed fermenter with sand. The degradation of 2-chloroethanol was connected with a release of protons, which led to a decrease of the pH in the medium. Higher initial concentration than 25 mM 2-chloroethanol were not degraded completely because they entailed a decrease of the pH to 5.0, which inhibited further growth and degradation. P. putida US2 showed a typical repression of catabolites and diauxic growth with succinate as cosubstrate. The addition of succinate as a second substrate caused a decrease in degradation of 2-chloroethanol. Activated sludge added to adsorbed cultures in a continuous fermentation did not lead to a decrease in metabolic activity. After 2 weeks of continuous cultivation the specialized strain could be retained.

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References

  • Balfanz J, Rehm H-J (1991) Biodegradation of 4-chlorophenol by adsorptive immobilized Acaligenes sp. A 7–2 in soil. Appl Microbiol Biotechnol 35:662–668

    CAS  PubMed  Google Scholar 

  • Bergmann JG, Sanik J (1957) Determination of trace amounts of chlorine in naphtha. Anal Chem 29:241–243

    Google Scholar 

  • Bettmann H, Rehm H-J (1985) Continuous degradation of phenol(s) by Pseudomonas putida P8 entrapped in polyacryamid-hydrazide. Appl Microbiol Biotechnol 20:389–393

    Google Scholar 

  • Clark PH, Richmond HH (1975) Genetics and biochemistry of Pseudomonas. J Wiley, London New York

    Google Scholar 

  • Greer CW, Beaumier D, Sammson R (1989) Application of on-line sensors during growth of the dichloroethane degrading bacterium X anthobacter autotrophicus. J Biotechnol 12:261–274

    Google Scholar 

  • Hofmann K (1987) Technischer Anforderungen an den Umgang mit CKW aus technischer Sicht. Vortrag zum Lehrgang 9240/11.028 an der technischen Akademie Esslingen am 09./10.03.1987

  • Janssen DB, Scheper A, Witholt B (1984) Biodegradation of 2-chloroethanol and 1,2-dichloroethane by pure bacterial cultures. In: Houwink EH, Vanden Meer RR (eds) Innovations in biotechnology. Elsevier, Amsterdam, pp 169–179

    Google Scholar 

  • Janssen DB, Scheper A, Djihuizen L, Witholt B (1985) Degradation of halogenated aliphatic compounds by X anthobacter autotrophicus GJ 10. Appl Environ Microbiol 49:673–677

    Google Scholar 

  • Janssen DB, Keuning S; Witholt B (1987) Involvement of a quinoprotein alcohol dehydrogenase and a NAD dependent aldehyde dehydrogenase in 2-chloroethanol-metabolism in X anthobacter autotrophicus GJ 10. J Gen Microbiol 133:85–92

    Google Scholar 

  • Knippschild M, Rehm HJ (1995) Degradation of 2-chloroethanol by free and immobilized Pseudomonas putida US2. Appl Microbiol Biotechnol (in press)

  • Matin A, Konings WN (1973) Transport of lactate and succinate by membrane vesicles of Escherichia coli, Bacillus subtilis and a Pseudomonas species. Eur J Biochem 34:58–67

    Google Scholar 

  • Omori T, Kimura T, Kodama T (1987) Bacterial cometabolic degradation of chlorinated paraffins. Appl Microbiol Biotechnol 25:533–557

    Google Scholar 

  • Scholz-Muramatsu H, Flemming HC (1992) Unter welcehn Bedingungen können leichtflüchtige CKW in der Natur abgebaut werden. In: Biologischer Abbau von Chlorkohlenwasserstoffen: Kolloquium an der TU Berlin, 21–22 November 1991, Technische Universität, Berlin. pp 163–185

    Google Scholar 

  • Steinhäuser KG (1987) Bewertung der CKW aus wasserwirtschaftlicher Sicht Vortrag zum Lehrgang 9240/11.028 an der technischen Akademie Esslingen, 09./10.3.1987

  • Strotmann U, Petenga M, Janssen DB (1990) Degradation of 2-chloroethanol by wild type and mutants of Pseudomonas putida US 2. Arch Microbiol 154:294–300

    Google Scholar 

  • Werner P (1991) Praktische Erfahrung mit der mikrobiologischen Bodensanierung — in situ — Verfahren. In: Behrens D, Wiesner J (eds) Mikrobiologische Reinigung von Böden: Beiträge des 9. DECHEMA-Fachgesprächs Umweltschutz am 27./28.02.1991 in Frankfurt am Main. DECHEMA, Frankfurt am Main; VCH, Weinheim, pp 181–203

    Google Scholar 

  • Westmeyer F, Rehm H-J (1987) Degradation of 4-chlorophenol in municipal wastewater by adsorptive immobilized Alcaligenes sp. A 7–2. Appl Microbiol Biotechnol 26:78–83

    Google Scholar 

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Authors and Affiliations

  1. Institut für Mikrobiologie, Universität Münster, Corrensstr. 3, D-48149, Münster, Germany

    C. Overmeyer & H.-J. Rehm

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  1. C. Overmeyer
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  2. H.-J. Rehm
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Overmeyer, C., Rehm, HJ. Biodegradation of 2-chloroethanol by freely suspended and adsorbed immobilized Pseudomonas putida US2 in soil. Appl Microbiol Biotechnol 43, 143–149 (1995). https://doi.org/10.1007/BF00170636

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  • DOI: https://doi.org/10.1007/BF00170636

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