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Cellobiose-quinone oxidoreductase —application in monitoring cellobiohydrolase purification

  • Applied Microbiology
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Summary

The enzymatic saccharification of cellulose has traditionally been monitored via total reducing sugar analyses. Yet with cellobiohydrolase being a major component of fungal and actinomycete cellulases, there is a need for a more specific measurement of cellobiose besides other soluble cellulodextrin products without interference from glucose, the major product of cellulose saccharification. One approach is to utilize cellobiose: quinone oxidoreductase (CBOase) from the fungus Phanerochaete chrysosporium to monitor saccharification. We describe here methods for the production of CBQase and a specialized application to monitor the chromatographic separation of cellobiohydrolase.

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Author notes

  1. Thomas J. Kelleher

    Present address: Cooper Technicon Inc., 19355, Malvern, Pennsylvania, USA

  2. Bland S. Montenecourt

    Present address: Department of Biology, Lehigh University, 18015, Bethlehem, Pennsylvania, USA

Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Cook College, Rutgers — The State University of New Jersey, 08903, New Brunswick, New Jersey, USA

    Thomas J. Kelleher, Bland S. Montenecourt & Douglas E. Eveleigh

Authors
  1. Thomas J. Kelleher
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  2. Bland S. Montenecourt
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  3. Douglas E. Eveleigh
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Dedicated to Professor Dr. H. J. Rehm on the occasion of his 60th birthday

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Kelleher, T.J., Montenecourt, B.S. & Eveleigh, D.E. Cellobiose-quinone oxidoreductase —application in monitoring cellobiohydrolase purification. Appl Microbiol Biotechnol 27, 299–305 (1987). https://doi.org/10.1007/BF00252934

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  • DOI: https://doi.org/10.1007/BF00252934

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