Abstract
Comparison of the nucleotide sequences of the C.C58 M75 myeloma ϰ chain gene and the BALB/c germ-line Jϰ segments suggested that the Jϰ regions of C.C58 and BALB/c might be distinguished by restriction enzyme polymorphisms. This was shown to be the case in Southern hybridizations of Hinf I and Ace I digests of liver DNA from these and other strains with a Jϰ-specific probe. Tests of a wide variety of inbred, congenic, recombinant, and recombinant-inbred strains provided evidence for three alleles, Igk-J a, Igk-J b, and Igk-J c, the type strains for which are C58/J, BALB/c, and SJL/J, respectively. Analysis of the B6.PL(85NS) congenic strain suggests that the Igk-J locus lies in the neighborhood of the Lyt-2/Lyt-3 loci, approximately 0.30 cM from the V gene segment determining the Igk-VSer and Igk-Efl polymorphisms. Finally, nucleotide substitutions lead to amino acid sequence differences between the C.C58 M 75 ϰ gene and the BALB/c germ line in Jϰ2 and Jϰ4. Two of these substitutions reflect true germ-line differences, raising the possibility that idiotype differences observed among strains could reflect Jϰ as well as Vϰ differences.
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Boyd, R.T., Goldrick, M.M. & Gottlieb, P.D. Genetic polymorphism at the mouse immunoglobulin J ϰ locus (Igk-J) as demonstrated by southern hybridization and nucleotide sequence analysis. Immunogenetics 24, 150–157 (1986). https://doi.org/10.1007/BF00364742
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DOI: https://doi.org/10.1007/BF00364742