Abstract
A previously undescribed subfamily of mouse class I MHC genes, consisting of two to three members, has been identified. The structure and organization of one of these, Mb1, has been determined. Mb1, consists of five exons with open reading frames and potentially encodes a class I-like transmembrane protein. In the genome, Mb1 is linked to the H-2 complex, mapping telomeric to Qa. However, this gene has low (ca. 60%) nucleotide identity with other class I sequences and is no more related to mouse class I genes than to class I genes from other species. Mb1 transcripts have not been found in a variety of adult tissues or cell lines, suggesting that, if Mb1 is expressed, its expression is highly regulated. From DNA sequence identity and intron-exon organization, Mb1 appears to be a primordial gene which antedates mouse speciation and which has evolved independently of the rest of the class I gene family. Examination of various species of wild mice demonstrates the presence of a discrete Mb1 subfamily over long evolutionary periods of time.
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Berk, A. and Sharp, P.: Sizing and mapping of early adenovirus mRNA's by electrophoresis of S1 endonuclease digested hybrids. Cell 12: 721–732, 1977
Collins, T., Lapierre, L. A., Fiers, W., Strominger, J. L., and Pober, J. S.: Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular en dothelial cells and dermal fibroblasts in vitro. Proc. Natl. Acad. Sci. U.S.A. 83: 446–450, 1986
Dale, R. M., McClure, B. A., and Houchins, J. P.: A rapid singlestranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 rDNA. Plasmid 13: 31–40, 1985
Devlin, J., Weiss, E. H., Paulson, M., and Flavell, R. A.: Duplicated gene pairs and alleles of class I genes in the Qa2 region of the murine MHC: a comparison. EMBO J. 12: 3203–3207, 1985
Ehrlich, R., Lifshitz, R., Pescovitz, M., Rudikoff, S., and Singer, D. S.: Tissue specific expression and structure of a different member of a class I MHC gene family. J. Immunol. 139: 539–602, 1987a
Ehrlich, R., Maguire, J., and Singer, D. S.: Identification of negative and positive regulatory elements associated with a class I MHC gene. in Mol. Cell. Biol. 8: 695–703, 1987b
Evans, G., Margulies, D., Camerini-Otero, R. D., Ozato, K., and Seidman, J.: Structure and expression of a mouse MHC gene, H-2Ld. Proc. Natl. Acad. Sci. U.S.A. 79: 1994–1998, 1982
Lindhal, P., Leary, P., and Gresser, I.: Enhancement by interferon of the expression of histocompatibility antigens of mouse lymphoid cells. Eur. J. Immunol. 4: 779–784, 1973
Malissen, M., Malissen, B., and Jordan, B.: Exon/intron organization and complete nucleotide sequence of an HLA gene. Proc. Natl. Acad. Sci. U.S.A. 79: 893–897, 1982
Maniatis, T., Fritsch, E. G., and Sambrook, J.: Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Mellor, A.: The class I MHC gene family in mice. Immunol. Today 7: 19–24, 1986
Mellor, A., Weiss, E., Ramachrandran, K., and Flavell, R.: A potential donor gene for the bml gene conversion event in the C57BL mouse. Nature 306: 792–794, 1983
Moore, K., Sher, B., Sun, Y., Eakle, K., and Hood, L.: DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen. Science 215: 679–682, 1982
O'Neill, A. E., Reid, K., Garberi, J. C., Karl, M., and Flaherty, L.: Extensive deletions in the Q region of the mouse major histocompatibility complex. Immunogenetics 24: 368–373, 1986
Pease, L., Schulze, D., Pfaffenbach, G., and Nathenson, S.: Spontaneous H-2 mutants provide evidence that a copy mechanism analogous to gene conversion generates polymorphism in the MHC. Proc. Natl. Acad. Sci. U.S.A. 80: 242–246, 1983
Ploegh, H. L., Orr, H. T., and Strominger, J. L.: Molecular cloning of a human histocompatibility antigen cDNA fragment. Proc. Natl. Acad. Sci. U.S.A. 77: 6081–6085, 1980
Pontarotti, P. A., Mashimo, H., Zeff, R. A., Fisher, D. A., Hood, L., Mellor, A., Flavell, R. A., and Nathenson, S. G.: Conservation and diversity in the class I genes of the major histocompatibility complex: sequence analysis of a Tlab gene and comparison with a Tlac gene. Proc. Natl. Acad. Sci. U.S.A. 83: 1782–1786, 1986
Queen, C. and Korn, L. J.: A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 12: 591–599, 1984
Rogers, M. J., Germain, R. N., Hare, J., Long, E., and Singer, D. S.: Comparison of MHC genes among distantly related members of the genus Mus. J. Immunol. 134: 630–636, 1985
Sanger, R., Nickles, S., and Colson, A.: DNA sequencing with chainterminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74: 5463–5467, 1977
Satz, M., Wang, L. C., Singer, D. S., and Rudikoff, S.: Structure and expression of two porcine genomic clones encoding class I MHC antigens. J. Immunol. 135: 2167–2175, 1985
Sodoyer, R., Damotte, M., Delovitch, T., Trucy, J., Jordan, B., and Strachan, T.: Complete nucleotide sequence of the gene encoding a functional human class I histocompatibility antigen (HLA-Cw3). EMBO J. 3: 879, 1984
Steinmetz, M., Moore, K. W., Frelinger, J., Sher, B. T., Shen, F., Boyse, E. A., and Hood, L.: A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell 25: 683–692, 1981
Steinmetz, M., Winoto, A., Minard, K., and Hood, L.: Clustering of genes encoding mouse transplantation antigens. Cell 28: 489–498, 1982
Strachan, T., Sodoyer, R., Damotte, M., and Jordan, B.: Complete nucleotide sequence of a functional class I HLA gene, HLA-A3; implications for the evolution of HLA genes. EMBO J. 3: 887–897, 1984
Transy, C., Nash, S. R., David-Watine, B., Cochet, M., Hunt, S. W., III, Hood, L. E., and Kourilsky, P.: A low polymorphic mouse H-2 class I gene from the Tla complex is expressed in a broad variety of cell types. J. Exp. Med. 166: 341–361, 1987
Warner, C. M., Gollnick, S. O., and Goldbard, S. B.: Linkage of the preimplantation-embryo-development (Ped) gene to the mouse major histocompatibility complex (MHC). Biol. Reprod. 36: 606–610, 1987a
Warner, C. M., Gollnick, S. O., Flaherty, L., and Goldbard, S. B.: Analysis of Qa-2 antigen expression by preimplantation mouse embryos: possible relationship to the preimplantation-embryo-de velopment gene (Ped) gene product. Biol. Reprod. 36: 611–616, 1987b
Weiss, E. H., Golden, L., Zakut, R., Mellor, A., Fahrner, K., Kvist, S., and Flavell, R. A.: The DNA sequence of the H-2I b gene; evidence for gene conversion as a mechanism for the generation of a polymorphism in histocompatibility antigens. EMBO J. 3: 453–462, 1983
Weiss, E. H., Golden, L., Fahmer, K., Mellor, A., Devlin, H., Tiddens, J., Bud, H., and Flavell, R. A.: Organization and evolution of the class I gene family in the MHC of the C57BL/10 mouse. Nature 310: 650–655, 1984
Wilbur, W. and Lipman, D.: Rapid similarity searches of nucleic acid and protein data banks. Proc. Natl. Acad. Sci. U.S.A. 80: 726–730, 1983
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Singer, D.S., Hare, J., Golding, H. et al. Characterization of a new subfamily of class I genes in the H-2 complex of the mouse. Immunogenetics 28, 13–21 (1988). https://doi.org/10.1007/BF00372524
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DOI: https://doi.org/10.1007/BF00372524