Abstract
Absorption of proteins by pinocytosis was studied in the postvalvular intestine of larvae of the sea bassDicentrarchus labrax during transition to the exotrophic phase. Comparison of specimens ranging in age from 4 or 5 d (age of mouth opening) to 25 d (period of strictly exotrophic feeding) made it possible to determine the morphofunctional development of enterocytes when fish are fed onArtemia sp. (Brazil strain). The pinocytotic activity of enterocytes was weak at the time of mouth opening, but intensified rapidly. After the first feed (7 to 8 d), enterocytes were capable of absorbing proteins, since they actively incorporated ferritin. At the age of 18 to 19 d, they showed a strong capacity for absorption resulting in the formation of a large supranuclear vacuole by the age of 25 d. Examination of the intestine of larvae fed artificially showed that enterocytes developed little until 18 to 19 d of age, but the presence of supranuclear vacuoles in the cytoplasm nevertheless indicated a capacity for absorption.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Alliot, E., Pastouraud, A., Trellu, J. (1977). Evolution des activités enzymatiques dans le tractus digestif au cours de la vie larvaire du bar,Dicentrarchus labrax. Variations des protéinogrammes et des zymogrammes. Publs Cent. natn. Exploit. Océans (CNEXO) (Sér. Act. Colloques) 4: 85–91
Bergot, P., Fléchon, J. E. (1970). Forme et voie d'absorption intestinale des acides gras à chaine longue chez la truite arc-en-ciel (Salmo gairdneri Rich.). II. Lipides “étalés”. Annls Biol. anim. Biochim. Biophys. 10: 473–480
Connes, R., Benhalima, K. (1983). Ultrastructure de l'intestin du loupDicentrarchus labrax L. au cours du développement larvaire. Bull. Soc. zool. Fr. 109: 19–33
Cornell, R., Padykula, H. A. (1969). A cytological study of intestinal absorption in the suckling rat. Am. J. Anat. 125: 291–316
Deplano, M. (1989). Etude morpho-fonctionnelle de la muqueuse intestinale du loup,Dicentrarchus labrax L. Mise en évidence de pathologies nutritionnelles chez les sujets immatures et chez les larves. Thèse. Université de Montpellier II, Sciences et Techniques du Languedoc, France
Deplano, M., Diaz, J. P., Connes, R., Kentouri-Divanach, M., Cavalier, F. (1991). Appearance of lipid-absorption capacities in larvae of the sea bassDicentrarchus labrax during transition to the exotrophic phase. Mar. Biol. 108: 361–371
Gauthier, G. F., Landis, S. C. (1972). The relationship of ultrastructural and cytochemical features to absorptive activity in the goldfish intestine. Anat. Rec. 172: 675–702
Gonnella, P. A., Neutra, M. R. (1984). Membrane-bound and fluidphase macromolecules enter separate prelysosomal compartments in absorptive cells of suckling rat ileum. J. Cell Biol. 99: 909–917
Govoni, J. J. (1980). Morphological, histological and functíonal aspects of alimentary canal and associated organ development in larvalLeiostomus xanthurus. Revue can. Biol. 39: 69–80
Loewe, H., Eckmann, R. (1988). The ontogeny of the alimentary tract of coregonid larvae: normal development. J. Fish Biol. 33: 841–850
Noaillac-Depeyre, J., Gas, N. (1973). Absorption of protein macromolecules by the enterocytes of the carp (Cyprinus carpio L.). Ultrastructural and cytochemical study. Z. Zellforsch. 146: 525–541
Noaillac-Depeyre, J., Gas, N. (1976). Electron microscopy study on gut epithelium of the tench (Tinca tinca L.) with respect to its absorptive functions. Tissue Cell 8: 511–530
Pearse, A. G. E. (1968). Histochemistry, theoretical and applied. Churchill Livingstone, London & New York
Quessada, J. (1987). Aspects lipidiques des développements embryonnaires et larvaires du loup,Dicentrarchus labrax, du sar,Diplodus sargus, et de la daurade,Sparus auratus, en relation avec les réserves endogènes et les conditions trophiques. Thèse Université de Montpellier II, Sciences et Techniques du Languedoc, France
Rombout, J. H. W. M., Sroband, H. W. J., Taverne-Thiele, J. J. (1984). Proliferation and differentiation of intestinal cells during development ofBarbus conchonius (Teleostei, Cyprinidae). Cell Tissue Res 236: 207–216
Sire, M. F., Vernier, J. M. (1981). Etude ultrastructurale de la synthèse de chylomicrons au cours de l'absorption intestinale des lipides chez la truite. Influence de la nature des acides gras ingéres. Biol. cell. 40: 47–62
Stroband, H. W. J., Dabrowski, K. R. (1979). Morphological and physiological aspects of the digestive system and feeding in fresh-water fish larvae. In: Fontaine, M. (ed.) Nutrition des poissons, Centre National de la Recherche Scientifique, Paris, p. 355–376 (Act. Colloques CNERMA)
Stroband, H. W. J., Kroon, A. G. (1981). The development of the stomach inClarias lazera and the intestinal absorption of macromolecules. Cell Tissue Res 215: 397–415
Stroband, H. W. J., Meer, H., v.d., Timmermans, L. P. M. (1979). Regional functional differentiation in the gut of the grasscarp,Ctenopharyngodon idella (Val.), Histochemistry 64: 235–249
Stroband, H. W. J., van der Veen, F. H. (1981). The localization of protein absorption during transport of food in the intestine of the grasscarp,Ctenopharyngodon idella (Val.). J. exp. Zool. 218: 149–156
Tanaka, M. (1972a). Studies on the structure and function of the digestive system in teleost larvae. IV. Changes in the epithelium related to fat absorption in the anteromedian part of the intestine after feeding. Jap. J. Ichthyol. 19: 15–25
Tanaka, M. (1972b). Studies on the structure and function of the digestive system in teleost larvae. V. Epithelial changes in the posterior gut and protein ingestion. Jap. J. Ichthyol. 19: 172–180
Vu, T. T. (1976). Etude du développement du tube digestif des larves de bar,Dicentrarchus labrax (L.). Archs Zool. exp. gén. 117: 493–509
Vu, T. T. (1980). Etude histologique de l'épithélium du tube digestif du barDicentrarchus labrax (L.) au cours du développement post embryonnaire. Archs Zool. exp. gén. 121: 131–206
Vu, T. T. (1983). Etude histoenzymologique des activités protéasiques dans le tube digestif des larves et des adultes de barDicentrarchus labrax (L.). Aquaculture, Amsterdam 32: 57–69
Watanabe, Y. (1984). Morphological and functional changes in rectal epithelium cells of pond smelt during post-embryonic development. Bull. Jap. Soc. scient. Fish. 50: 805–814
Watanabe, Y., Sawada, N. (1985). Larval development of digestive organs and intestinal absorptive functions in the freshwater gobyChaenogobius annularis. Bull. Tohoku reg. Fish. Res. Lab. 47: 1–10
Author information
Authors and Affiliations
Additional information
Communicated by J.M. Pérès, Marseille
Rights and permissions
About this article
Cite this article
Deplano, M., Connes, R., Diaz, J.P. et al. Variation in the absorption of macromolecular proteins in larvae of the sea bassDicentrarchus labrax during transition to the exotrophic phase. Mar. Biol. 110, 29–36 (1991). https://doi.org/10.1007/BF01313089
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01313089