Summary
Cell walls of Schizochytrium aggregatum and Thraustochytrium sp. were mechanically isolated and subjected to chemical analysis. On a dry weight basis the cell walls contain 21–36% carbohydrate and 30–43% protein. The principal sugar (>95%) of the Schizochytrium wall is l-galactose, while the Thraustochytrium cell wall contains l-galactose, d-galactose and xylose with l-galactose predominating. Ultrastructurally the cell walls of both organisms consist of a laminated structure which yields thin, flexible, nearly circular scales (0.5–1.1 μ in diameter) upon sonic disintegration. Structures presumed to be developing wall scales are found within cisternae of the Golgi apparatus in both organisms. The chemical composition and method of formation of the cell wall in these two protists is distinctly different from that found in the Saprolegniales (Oomycetes), the group with which these organisms have hitherto been aligned.
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References
Aronson, J. M., Fuller, M. S.: Cell wall structure of the marine fungus, Atkinsiella dubia. Arch. Mikrobiol. 68, 295–305 (1969).
Avigad, G., Amaral, D., Asensio, C., Horecker, B. L.: The d-galactose oxidase of Polyporus circinatus. J. biol. Chem. 237, 2736–2743 (1962).
Bahnweg, G., Sparrow, F. K., Jr.: Aphanochytrium kergeulensis gen nov. spec. nov., a new phycomycete from subarctic marine waters. Arch. Mikrobiol 81, 45–49 (1972).
Bartnicki-Garcia, S.: Chemistry of hyphal walls of Phytophthora. J. gen. Microbiol. 42, 57–69 (1966).
Bartnicki-Garcia, S.: Cell wall composition and other biochemical markers in fungal phylogeny. In: J. B. Harborn (ed.), Phytochemical phylogeny, pp. 81–103. New York: Acad. Press 1970.
Brown, R. M., Jr.: Observations on the relationship of the Golgi apparatus to wall formation in the marine Chrysophycean alga, Pleurochrysis scherffelii Pringsheim. J. Cell Biol. 41, 109–123 (1969).
Brown, R. M., Jr., Franke, W. W., Kleinig, H., Falk, H., Sitte, P.: Scale formation in chrysophycean algae. I. Cellulosic and noncellulosic wall components made by the Golgi apparatus. J. Cell Biol. 45, 246–271 (1970).
Darley, W. M., Fuller, M. S.: Cell wall chemistry and taxonomic position of Schizochytrium. Amer. J. Bot. 57, 761 (1970).
Dill, B. C., Fuller, M. S.: Amino acid immobilization of fungal motile cells. Arch. Mikrobiol. 78, 92–98 (1971).
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., Smith, F.: Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28, 350–356 (1956).
Fuller, M. S., Fowles, B. E., McLaughlin, D. J.: Isolation and pure culture study of marine Phycomycetes. Mycologia (N.Y.) 56, 745–756 (1964).
Goldstein, S., Belsky, M.: Axenic culture studies of a new marine phycomycete possessing an unusual type of asexual reproduction. Amer. J. Bot. 51, 72–78 (1964).
Goldstein, S., Moriber, L., Hershenov, B.: Ultrastructure of Thraustochytrium aureum, a biflagellate marine phycomycete. Mycologia (N.Y) 56, 897–904 (1964).
Grove, S. N., Bracker, C. E., Morre, D. J.: An ultrastructural basis for hyphal tip growth in Pythium ultimum. Amer. J. Bot. 57, 245–266 (1970).
Heath, I. B., Gay, J. L., Greenwood, A. D.: Cell wall formation in the Saprolegniales: Cytoplasmic vesicles underlying developing walls. J. gen. Microbiol. 65, 225–232 (1971).
Kazama, F.: Ultrastructure of Thraustochytrium sp. zoospores. I. Kinetosome. Arch. Mikrobiol. 83, 179–188 (1972).
Kobayashi, Y., Ookubo, M.: Studies on the marine Phycomycetes. I. Bull. Nat. Sci. Mus. Tokyo 33, 53–65 (1953).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Percival, E., McDowell, R. H.: Chemistry and enzymology of marine algal polysaccharides. New York: Academic Press 1967.
Perkins, F. O.: The ultrastructure of holdfasts, “rhizoids”, and “slime tracks” in thraustochytriaceous fungi and Labyrinthula spp. Arch. Mikrobiol. 84, 95–118 (1972).
Perkins, F. O., Amon, J. P.: Zoosporulation in Labyrinthula sp.; an electron microscope study. J. Protozool. 16, 235–257 (1969).
Porter, D.: Ultrastructure of Labyrinthula. Protoplasma (Wien) 67, 1–19 (1969).
Randerath, K.: Thin-layer chromatography. New York: Academic Press 1964.
Sparrow, F. K.: The aquatic phycomycetes, exclusive of the Saprolegniaceae and Pythium. Ann Arbor: Univ. Michigan Press 1943.
Trotter, M. J., Whisler, H. C.: Chemical composition of the cell wall of Amoebidium parasiticum. Canad. J. Bot. 43, 869–876 (1965).
Turvey, J. R.: Sulfates of the simple sugars. Advanc. Carbohydrate Chem. 20, 183–218 (1965).
Watson, S. W.: Cultural and cytological studies on species of Labyrinthula. Ph.D. thesis, Univ. Wisconsin 1957.
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Darley, W.M., Porter, D. & Fuller, M.S. Cell wall composition and synthesis via Golgi-directed scale formation in the marine eucaryote, Schizochytrium aggregatum, with a note on Thraustochytrium sp.. Archiv. Mikrobiol. 90, 89–106 (1973). https://doi.org/10.1007/BF00414512
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DOI: https://doi.org/10.1007/BF00414512