Abstract
When cells of Synechocystis, adapted to 684 mmol/l NaCl, were exposed to hypoosmotic shock by reducing NaCl concentration to more than 250 mmol/l, significant amounts of organic material were liberated which could be increased by increasing shock strength. After maximal hypoosmotic shock (684 mmol/l→ 2 mmol/l NaCl) 40–50% of photosynthetically labelled organic material occurred in the surrounding medium. The main compound exudates was the osmoprotective compound glucosylglycerol. Minor exudates were amino acids, organic acids and carbohydrates. In contrast a hyperosmotic shock (2 mmol/l→684 mmol/l NaCl) liberated only one fourth of the amount liberated by a hypoosmotic shock.
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References
Allen MB, Arnon DJ (1955) Studies on nitrogen-fixing algae. II. The sodium requirement of Anabaena cylindrica. Physiol Plant 8: 653–660
Enhuber G, Gimmler H (1980) The glycerol permeability of the plasmalemma of the halotolerant green alga Dunaliella parva (Volvocales). J Phycol 16: 524–532
Erdmann N (1983) Organic osmoregulatory solutes in blue-green algae. Z Pflanzenphysiol 110: 147–155
Gilmour DJ, Hipkins MF, Boney AD (1984) The effect of decreasing salinity on the primary processes of photosynthesis in Dunaliella tertiolecta. J Exp Bot 35: 28–35
Hagemann M, Erdmann N, Wittenburg E (1987) Synthesis of glucosylglycerol in salt-stressed cells of the cyanobacterium Microcystis firma. Arch Microbiol 148: 275–279
Reed RH, Richardson DL, Stewart WDP (1985) Na+ uptake and extrusion in the cyanobacterium Synechocystis PCC 6714 in response to hypersaline treatment. Evidence for transient changes in plasmalemma Na+ permeability. Biochim Biophys Acta 814: 347–355
Reed RH, Borowitzka LJ, Mackay MA, Chudek JA, Foster R, Warr SRC, Moore DJ, Stewart WDP (1986a) Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiol Rev 39: 51–56
Reed RH, Warr SRC, Kerby NW, Stewart WDP (1986b) Osmotic shock-induced release of low molecular weight metabolites from free-living and immobilized cyanobacteria. Enzyme Microb Technol 8: 101–104
Tel-Or E, Spath S, Packer L, Mehlhorn RJ (1986) Carbon-13 NMR studies of salt shock-induced carbohydrate turnover in the marine cyanobacterium Agmenellum quadruplicatum. Plant Physiol 82: 646–652
Warr SRC, Reed RH, Chudek JA, Foster R, Stewart WDP (1985) Osmotic adjustment in Spirulina platensis. Planta 163: 424–429
Zidan MA, Hipkins MF, Boney AD (1987) Loss of intracellular glycerol from Dunaliella tertiolecta after decreasing external salinity. J Plant Physiol 127: 461–469
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Fulda, S., Hagemann, M. & Libbert, E. Release of glucosylglycerol from the cyanobacterium Synechocystis spec. SAG 92.79 by hypoosmotic shock. Arch. Microbiol. 153, 405–408 (1990). https://doi.org/10.1007/BF00249013
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DOI: https://doi.org/10.1007/BF00249013