Abstract
Spores of the thermophilic, acidophilic, Bacillus acidocaldarius were covered by a thick outer coat and a laminated inner coat (5.5 nm periodicity). Small membranous vesicles were present in the spore core and they disappeared as germination proceeded. After depolymerization of the cortex, and a 30% increase in spore diameter, a localized gap appeared in the laminated inner coat only. This inner coat gap was narrow and could be the whole length of the spore. The germ cell appeared to grow, or to be pushed towards the inner coat gap, at which stage the outer coat disappeared in the same localized area. As the vegetative cell grew out the spore coat fell away, with loose cortical material still attached to it. The young germ cell developed a large spherical electron dense inclusion body in the cytoplasm, at the same time as the ribosomal and nuclear areas became distinct.
References
Darland, G., Brock, T. D.: Bacillus acidocaldarius sp. nov., an acidophilic, thermophilic, spore-forming bacterium. J. gen. Microbiol. 67, 9–15 (1971)
De Rosa, M., Gambacorta, A., Minale, L., Bu'Lock, J.D.: A new biosynthetic pathway, the formation of cocyclohexyl fatty acids from shikimate in an acidophilic, thermophilic bacillus. Biochem. J. 128, 751–754 (1972)
Golovacheva, R.S.: Spontaneous generation of spherical forms in obligate thermophilic Bacilli. Mikrobiologiya 36, 667–674 (1967)
Hamilton, W.A., Stubbs, J.M.: Comparison of the germination and outgrowth of spores of Bacillus cereus and Bacillus polymyxa. J. gen. Microbiol. 47, 121–129 (1967)
Hashimoto, T., Conti, S.F.: Ultrastructural changes associated with activation and germination of Bacillus cereus T spores. J. Bact. 105, 361–368 (1971)
Kellenberger, E., Ryter, A., Séchaud, J.: Electron microscope study of DNA containing plasms. ii. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. biophys. biochem. Cytol. 4, 671–678 (1958)
Murray, R.G.E., Hall, M.M., Marak, J.: The maturation of cell wall structure during germination of Bacillus polymyxa spores. Canad. J. Microbiol. 16, 883–887 (1970)
Rousseau, M., Fléchon, J., Hermier, J.: Étude au microscope électronique, de la germination de la spore chez Bacillus subtilis. Ann. Inst. Pasteur 111, 149–160 (1966)
Spurr, A.R.: A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–34 (1969)
Strange, R.E., Hunter, J.R.: Outgrowth and synthesis of macromolecules. In: The bacterial spore, G. W. Gould, A. Hurst, eds., pp. 445–483. London-New York: Academic Press 1969
Walker, P.D., Baillie, A.: Structure of Bacillus stearothermophilus: An electron microscope study. J. appl. Bact. 31, 108–113 (1968)
Warth, A.D., Ohye, D.F., Murrell, W.G.: The composition and structure of bacterial spores. J. Cell Biol. 16, 579–592 (1963)
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Handley, P.S., Knight, D.G. Ultrastructural changes occurring during germination and outgrowth of spores of the thermophile Bacillus acidocaldarius . Arch. Microbiol. 102, 155–161 (1975). https://doi.org/10.1007/BF00428361
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DOI: https://doi.org/10.1007/BF00428361