Abstract
Mutants of Rhodopseudomonas capsulata lacking uptake hydrogenase activity have been isolated among those unable to grow photoautotrophically. Studies with these mutants showed increases in nitrogenase mediated H2 production from all substrates tested. In addition, photosynthetic synthetic growth on N2 with malate as carbon source was not affeced by the block in H2 uptake even under low light. Under these growth conditions hydrogen was observed to accumulate in mutant but not in wild-type cultures. This finding suggested that H2 was evolved by nitrogenase during N2 fixation by this photosynthetic bacterium and was efficiently recycled in the wild type.
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Takakuwa, S., Odom, J.M. & Wall, J.D. Hydrogen uptake deficient mutants of Rhodopseudomonas capsulata . Arch. Microbiol. 136, 20–25 (1983). https://doi.org/10.1007/BF00415604
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DOI: https://doi.org/10.1007/BF00415604