Abstract
Mitochondrial DNA polymorphism was employed to assess cytoplasmic diversity among cytoypes of the genus Cichorium and related genera of the tribe Lactuceae (Asteraceae). Hybridization patterns of total DNA using six restriction enzymes and five heterologous mtDNA probes were examined. From estimates of mtDNA diversity, Cichorium spinosum appeared as an ecotype of C. intybus rather than a separate species. Interspecific mtDNA polymorphism in the genus Cichorium was higher than that observed in Cicerbita Crepis, Lactuca and Tragopogon. Molecular data seemed to indicate that Catananche is very distant from the other genera examined. Intergeneric comparisons allowed the clustering of Cicerbita, Lactuca and Cichorium, genera which belong to different subtribes. However, further molecular investigations on a larger number of genera are needed to clarify the relationships among genera within and between subtribes of the tribe Lactuceae.
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References
Bellamy A (1992) Application du polymorphisme moléculaire de l'ADN à l'identification variétale de Cichorium intybus. Thèse, Université de Paris Xi-Orsay
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–402
Berthou F, Mathieu C, Vedel F (1983) Chloroplast and mitochondrial DNA variation as indicators of phylogenetic relationships in the genus Coffea L. Theor Appl Genet 65:77–84
Blackmore S (1981) Palynology and intergeneric relationships in the subtribe Hyoseridinae (Compositae: Lactuceae). Bot J Lin Soc 82:1–13
Bland MM, Matzinger DF, Levings CI (1985) Comparison of the mitochondrial genome of Nicotiana tabacum with its progenitor species. Theor Appl Genet 69:535–541
Bonnier G, De Layens G (1923). Flore complète portative de la France, de la Suisse et de la Belgique. Belin, Paris
Breiman A, Bogher M, Sternberg H, Graur D (1991) Variability and uniformity of mitochondrial DNA in populations of putative diploid ancestors of common wheat. Theor Appl Genet 82:201–208
Chowdhury MKU, Smith RL (1988) Mitochondrial DNA variation in pearl millet and related species. Theor Appl Genet 76:25–32
Dawson AJ, Jones VP, Leaver CJ (1984) The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J 3: 2107–2113
DeBonte LR, Matthews BF, Wilson KG (1984) Variation of plastid and mitochondrial DNAs in the genus Daucus. Am J Bot 71:932–940
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Derks FHM, Hakkert JC, Verbeek WHJ, Colijn-Hooymans CM (1992) Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction. Theor Appl Genet 84:930–940
Feinberg AP, Volgelstein B (1983) A technique for radiolabeling DNA restriction endonucleases fragments to high specific activity. Anal Biochem 132:6–13
Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56
Graur D, Bogher M, Breiman A (1989) Restriction endonuclease profiles of mitochondrial DNA and the origin of the B genome of bread-wheat Triticum aestivum. Heredity 62:335–342
Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461–486
Ichikawa H, Tanno-Suenaga L, Imamura J (1989) Mitochondrial genome diversity among cultivars of Daucus carota (ssp sativus) and their wild relatives. Theor Appl Genet 77:39–43
Jeffrey C (1966) Notes on Compositae. I. The Cichorieae in east tropical Africa. Kew Bull 18:427–486
Kemble RJ, Gunn RE, Flavell RB (1983) Mitochondrial DNA variation in races of maize indigenous to Mexico. Theor Appl Genet 65:129–144
Khairallah MM, Adams MW, Sears BB (1991) Mitochondrial genome size variation and restriction fragment length polymorphisms in three Phaesolus species. Theor Appl Genet 82:321–328
Khandjian EW (1987) Optimized hybridization signal of DNA blotted and fixed to nitrocellulose and nylon membranes. Bio/Technology 5:165–167
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
McClean PE, Hanson MR (1986) Mitochondrial DNA sequence divergence among Lycopersicon and related Solanum species. Genetics 112:649–667
Miller JC (1990) Concerning Restsite package v1.1. University of Wisconsin
Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273
Nugent JM, Palmer JD (1991) RNA-mediated transfer of the gene coxII from the mitochondrion to the nucleus during flowering plant evolution. Cell 66:473–481
Palmer JD, Hebron LA (1988) Plant mitochondrial DNA evolves rapidly in structure but slowly in sequence. J Mol Evol 28:87–97
Pelletier G, Primard C, Ferault M, Vedel F, Chétrit P, Renard M, Delourme R (1988) Use of protoplasts in plant breeding: cytoplasmic aspects. Plant Cell Tissue Org Cult 12:173–180
Perl A, Aviv D, Galun E (1991) Nuclear-organelle interaction in Solanum: interspecific cybridizations and their correlation with a plastome dendrogram. Mol Gen Genet 228:193–200
Sedoroff RR (1987) Molecular mechanism of mitochondrial genome evolution in higher plants. Am Nat 130:530–545
Sederoff RR, Levings III CS, Timothy DH, Hu WWL (1981) Evolution of DNA sequence organization in mitochondrial genomes of Zea. Proc Natl Acad Sci USA 78:5953–5957
Sneath PHA, Sokal RO (1973) Numerical taxonomy. Freeman, San Francisco
Stebbins GL (1953) A new classification of the tribe Cichorieae, family Compositae. Madrono 12:23–64
Stebbins GL, Jenkins JA, Walters MS (1953) Chromosomes and phylogeny in the Compositae, tribe Cichorieae. Univ Calif Publ Bot 16:401–430
Stern DB, Palmer JD (1984) Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci USA 81:1946–1950
Terachi T, Tsunewaki K (1992) The molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops. VIII. Mitochondrial RFLP analyses using cloned genes as probes. Mol Biol Evol 9:917–931
Timothy DH, Levings III CS, Pring DR, Conde MF, Kermicle JL (1979) Organelle DNA variation and systematic relationships in the genus Zea: Teosinte. Proc Natl Acad Sci USA 76:4220–4224
Tutin TG, Heywood VH, Burges NA, Valentine DH, Walters SM, Webb DA (1964–1980) Flora Europaea. Cambridge University Press, UK
Vedel F, Quetier F, Dosba F, Doussinault G (1978) Study of wheat phylogeny by EcoRI analysis of chloroplastic and mitochondrial DNAs. Plant Sci Lett 13:97–102
Wessinger AK, Timothy DH, III LCS, Goodman MM (1983) Patterns of mitochondrial DNA variation in indigenous maize races of Latin America. Genetics 104:365–379
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Vermeulen, A., Desprez, B., Lancelin, D. et al. Relationships among Cichorium species and related genera as determined by analysis of mitochondrial RFLPs. Theoret. Appl. Genetics 88, 159–166 (1994). https://doi.org/10.1007/BF00225892
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DOI: https://doi.org/10.1007/BF00225892