Summary
Five generations of divergent selection for plasma concentration of insulin-like growth factor-1 (IGF-1) and for 12-week body weight were carried out in mice, including randomly selected control lines for each trait. All lines were replicated once (12 lines in total). Each replicate line consisted of eight male and eight female parents per generation. Litter size was standardized to eight pups at birth. Mass selection was applied in the selected lines and within-family random selection in the control lines. Blood was taken from the orbital sinus of individual mice at 12 weeks of age for IGF-1 assay. Realized heritabilities were 0.10±0.01 for IGF-1 and 0.41 ± 0.02 for 12-week weight. The realized genetic correlation between IGF-1 and 12-week weight was 0.58 ± 0.01, with a phenotypic correlation of 0.38. Although the genetic correlation between IGF-1 and body weight in mice is moderately positive, 12-week weight responded 3.5 times as fast to weight selection as to selection for IGF-1.
Similar content being viewed by others
References
Blair HT, McCutcheon SW, Mackenzie DDS, Gluckman PD, Ormsby JE, Breier BH (1989) Responses to divergent selection for plasma concentrations of insulin-like growth factor-1 in mice. Genet Res 53:187–191
Breier BH, Bass JJ, Butler JH, Gluckman PD (1986) The somatotrophic axis in young steers: influence of nutritional status on pulsatile release of growth hormone and circulating concentrations of insulin-like growth factor-1. J Endocrinol 111:209–215
Breier BH, Gluckman PD, Bass JJ (1988a) Influence of nutritional status and oestrodial-17β on plasma growth hormone, insulin-like growth factors-I and -II, and the response to exogenous growth hormone in young steers. J Endocrinol 118:243–250
Breier BH, Gluckman PD, Bass JJ (1988b) Plasma concentrations of insulin-like growth factor-1 and insulin in the infant calf: ontogeny and influence of altered nutrition. J Endocrinol 119:43–50
Canalis E (1980) Effect of insulin-like growth factor-I on DNA and protein synthesis in cultured rat calvarie. J Clin Invest 66:709–719
Clemmons DR, Van Wyk JJ (1981) Somatomedin: physiological control and effects on cell proliferation. In: Basearga R (ed) Tissue growth factors. Handbook of experimental pharmacology. Springer, Berlin, pp 161–208
Clemmons DR, Thissen JP, Maes M, Ketelslegers JM, Underwood LE (1989) Insulin-like growth factor-1 infusion into hypophysectomized or protein-deprived rats induces specific IGF binding proteins in serum. Endocrinology 125:2969–2972
D'Ercole AJ, Stiles AD, Underwood EE (1984) Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc Natl Acad Sci USA 81:935–939
Daughaday WH, Rotwein P (1989) Insulin-like growth factors-I and -II. Peptide, messenger ribonucleic acid and gene structures, serum and tissue concentrations. Endocrinol Rev 10:68–91
Eigenmann JE, Patterson DF, Froesch ER (1984) Body size parallels insulin-like growth factor-I levels but not growth hormone secretary capacity. Acta Endocrinol 106:448–453
Falconer DS (1954) Validity of the theory of genetic correlation. J Hered 45:42–44
Falconer DS (1973) Replicated selection for body weight in mice. Genet Res 22:291–321
Falconer DS (1981) Introduction to quantitative genetics, 2nd edn. Longman, London New York, 340 pp
Gluckman PD, Butler JH (1983) Parturition-related changes in the insulin-like growth factors-1 and -2 in the perinatal lamb. J Endocrinol 99:223–232
Gluckman PD, Breier BH, Davis SR (1987) Physiology of the somatotropic axis with particular reference to the ruminant. J Dairy Sci 70:442–466
Handelsman DJ, Spaliviero JA, Scott DC, Baxter RC (1987) Hormonal regulation of the peripubertal surge of insulin-like growth factor-1 in the rat. Endocrinology 120:491–496
Kachman SD, Baker RL, Gianola D (1988) Phenotypic and genetic variability of estimated growth curve parameters in mice. Theor Appl Genet 76:148–156
Lund-Larsen TR, Bakke H (1975) Growth hormone and somatomedin activities in lines of pigs selected for rate of gain and thickness of backfat. Acta Agric Scand 25:231–234
McCarthy JC (1982) The laboratory mouse as a model for animal breeding: a review of selection for increased body weight and litter size. Proc 2nd World Congr Genet Appl Livest Prod 5:66–83
Monier S, Le Cam A, Le Marchand-Brustel Y (1983) Insulin and insulin-like growth factor-I. Effects on protein synthesis in isolated muscles from len and goldthioglucose-obese mice. Diabetes 32:392–397
Ooi GT, Herington AC (1988) The biological and structural characterization of specific serum binding proteins for insulin-like growth factors. J Endocrinol 118:7–18
Schmid C, Steiner I, Froesch ER (1984) Insulin-like growth factor-I supports differentiation of cultural osteoblast-like cells. FEBS Lett 173:48–52
Schoenle E, Zapf J, Humbel RE, Froesch ER (1982) Insulin-like growth factor-I stimulates growth in hypophysectomized rats. Nature 296:252–253
Zapf J, Schmid C, Froesch ER (1984) Biological and immunological properties of insulin-like growth factors (IGF) I and II. Clin Endocrinol Metab 13:3–30
Zapf J, Hauri C, Waldvogel M, Futo E, Hasler H, Binz K, Guler HP, Schmid C, Froesch ER (1989) Recombinant human insulin-like growth factor-1 induces its own specific carrier protein in hypophysectomized and diabetic rats. Proc Natl Acad Sci USA 86:3813–3817
Author information
Authors and Affiliations
Additional information
Communicated by D. Van Vleck
Rights and permissions
About this article
Cite this article
Baker, R.L., Peterson, A.J., Bass, J.J. et al. Replicated selection for insulin-like growth factor-1 and body weight in mice. Theoret. Appl. Genetics 81, 685–692 (1991). https://doi.org/10.1007/BF00226738
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00226738