Summary
A full-length, low-molecular-weight (LMW) glutenin cDNA clone, pTdUCD1, has been isolated from a Triticum durum cv ‘Mexicali’ wheat cDNA library. The complete sequence was determined and compared to the LMW glutenin genes that have been isolated from hexaploid wheat, Triticum aestivum. This cDNA codes for a protein of 295 amino acids (33,414 daltons) including a 20-amino acid signal peptide as deduced from the DNA sequence. Northern analysis showed that this cDNA hybridizes to a family of related sequences ranging in length from 1,200 to 1,000 nucleotides. This gene is similar but not identical to previously published LMW glutenin gene sequences. The most striking characteristic of all cloned LMW glutenin genes is the conservation of eight cysteine residues, which could be involved in potential secondary or tertiary structure, disulfide bond interactions. This paper presents a structural map defining distinct regions of the LMW glutenin gene family.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alexander DC (1987) An efficient vector-primer cDNA cloning system. In: Wu R, Grossman L (eds) Methods in enzymology, vol 154. Academic Press, New York, pp 44–64
Ausubel FM, Brent R, Kingston RE, Moore DD, Siedman JG, Smith JA, Struhl K (1987) Current methods in molecular biology. Greene Publishing Associates and Wiley Interscience, New York
Bartels D, Thompson RD (1983) The characterization of cDNA clones coding for wheat storage proteins. Nucleic Acids Res 11:2961–2977
Branlard G, Dardevet M (1985) Diversity of grain proteins and bread wheat quality. II. Correlation between high-molecular-weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354
Colot V, Bartels D, Thompson R, Flavell R (1989) Molecular characterization of an active wheat LMW glutenin gene and its relation to other wheat and barley prolamin genes. Mol Gen Genet 216:81–90
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Grunstein M, Hogness DS (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965
Gupta RB, Shepherd KW (1988) Low-molecular-weight glutenin subunits in wheat: their variation, inheritance, and association with bread-making quality. In: TE Miller, RMD Koebner (eds) 7th Int Wheat Genet Symp Inst Plant Sci Res, pp 943–949
Gupta RB, Singh NK, Shepherd KW (1989) The cumulative effect of allelic variation in LMW and HMW glutenin subunits on dough properties in the progeny of two bread wheats. Theor Appl Genet 77:57–64
Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197
Kasarda DD, Tao HP, Evans PK, Adalsteins AE, Yuen SW (1988) Sequencing of a protein from a single spot of a 2-D gel pattern: N-terminal sequence of a major wheat LMW-glutenin subunit. J Exp Bot 39:899–906
Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley, and rye. Oxford Surv Plant Mol Cell Biol 2:253–317
Lagudah S, MacRitchie F, Halloran GM (1987) The influence of high-molecular-weight subunits of glutenin from Triticum tauschii on flour quality of synthetic hexaploid wheat. J Cereal Sci 5:129–138
Moonen JHE, Zeven AC (1985) Association between highmolecular-weight subunits of glutenin and bread-baking quality in wheat lines derived from backcrosses between Triticum aestivum and Triticum speltoides. J Cereal Sci 3:97–101
Okita TW (1984) Identification and DNA sequence analysis of a gamma-type gliadin cDNA plasmid from winter wheat. Plant Mol Biol 3:325–332
Okita TW, Chessbrough V, Reeves CD (1985) Evolution and heterogeneity of the α/β-type sequences and γ-type gliadin DNA sequences. J Biol Chem 260:8203–8213
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Corfield KG, Blackman JA (1979) Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theor Appl Genet 55:153–159
Payne PI, Corfield KG, Holt LM, Blackman JA (1981) Correlations between the inheritance of certain high-molecularweight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60
Pitts EG, Rafalski JA, Hedgcoth C (1988) Nucleotide sequence and encoded amino acid sequence of a genomic gene region for a low-molecular-weight glutenin. Nucleic Acids Res 16:11376
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shepherd KW (1988) Genetics of wheat endosperm proteins — in retrospect and prospect. In: Miller TE, Koebner RMD (eds) 7th Int Wheat Genet Symp Inst Plant Sci Res, pp 919–931
Tao P, Kasarda DD (1989) 2-dimensional gel mapping and N-terminal sequencing of LMW glutenin subunits. J Exp Bot 40:1015–1020
Wall JS (1979) The role of wheat proteins in determining baking quality. In: Laidman DL, Wyn Jones RG (eds) Recent advances in the biochemistry of cereals. Academic Press, pp 273–311
Author information
Authors and Affiliations
Additional information
Communicated by A. L. Kahler
Rights and permissions
About this article
Cite this article
Cassidy, B.G., Dvorak, J. Molecular characterization of a low-molecular-weight glutenin cDNA clone from Triticum durum . Theoret. Appl. Genetics 81, 653–660 (1991). https://doi.org/10.1007/BF00226733
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00226733