Abstract
The somatic cell hybrid C121, with chromosome 7 as its sole human component, arose when mouse macrophages were immortalized by fusion with SV40-transformed human fibroblasts. The transforming SV40 genomes are integrated at 7q31-7q35. We show that hybrids with a reduced chromosome 7 component, but which retain markers linked to the cystic fibrosis locus, can be generated by direct in vivo tumor selection or following chromosome-mediated gene transfer and SV40-mediated cellular transformation. Our methods for chromosome fragmentation and fine-structure mapping can now be applied to the substantial number of SV40-transformed human cell lines, with independent chromosomal integration sites, already available. Our results also suggest that expression of human epidermal growth factor receptor augments the tumorigenic potential of the SV40-transformed C121 hybrid.
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Literature cited
Orkin, S.H. (1986).Cell 47845–850.
Porteous, D.J. (1987).Trends Genet. 3177–182.
Porteous, D.J., Morten, J.E.N., Cranston, G., Fletcher, J.M., Mitchell, A., van Heyningen, V., Fantes, J.A., Boyd, P.A., and Hastie, N.D. (1986).Mol. Cell. Biol. 62223–2232.
Porteous, D.J., Morten, J.E.N., Foster, M.E., Cranston, G., Weir-Thompson, E., Busuttil, A., Bostock, C.J., and Steel, C.M. (1986).Int. J. Cancer 38602–612.
Porteous, D.J., Bickmore, W., Christie, S., Boyd, P.A., Cranston, G., Fletcher, J.M., Gosden, J.R., Rout, D., Seawright, A., Simola, K.O.J., van Heyningen, V., and Hastie, N.D. (1987).Proc. Natl. Acad. Sci. U.S.A. 845355–5359.
Bickmore, W., Christie, S., van Heyningen, V., Hastie, N.D., and Porteous, D.J. (1988).Nucleic Acid Res. 1651–60.
White, R., Woodward, S., Leppert, M., O'Connell, P., Hoff, M., Herbst, J., Lalouel, J.M., Dean, M., and vande Woude, G. (1985).Nature 318382–384.
Porteous, D.J., and van Heyningen, V. (1986).Trends Genet. 2149–152.
Park, M., Dean, M., Cooper, C.S., Schmidt, M., O'Brien, S.J., Blair, D.G., and vande Woude, G.F. (1986).Cell 45895–904.
Tempest, P.R., Reeves, B.R., Spurr, N.K., Rance, A.J., Chan, A.M.L., and Brookes, P. (1986).Carcinogenesis 72051–2057.
Scambler, P.J., Law, H.Y., Williamson, R., and Cooper, C.S. (1986).Nucleic Acid Res. 47159–7174.
Estivill, X., Farrall, M., Scambler, P.J., Bell, G.M., Hawley, K.M.F., Lench, N.J., Bates, G.P., Kruyer, H.C., Frederick, P.A., Stanier, P., Watson, E.K., Williamson, R., and Wainwright, B.J. (1987).Nature 326840–845.
Croce, C.M., and Koprowski, H. (1974).J. Exp. Med. 1401221–1229.
Mitchell, A.R., Ambros, P., Gosden, J.R., Morten, J.E.N., and Porteous, D.J. (1986).Somat. Cell Mol. Genet. 12313–324.
Campo, M.S., Cameron, I.R., and Rogers, M.E. (1978).Cell 151411–1426.
van Heyningen, V., Boyd, P.A., Seawright, A., Fletcher, J.M., Fantes, J.A., Buckton, K.E., Spowart, G., Porteous, D.J., Hill, R.E., Newton, M.S., and Hastie, N.D. (1985).Proc. Natl. Acad. Sci. U.S.A. 828592–8596.
Waterfield, M.D., Mayes, E.L.V., Stroobant, P., Bennet, P.L.P., Young, S., Goodfellow, P.N., Banting, G.S., and Ozanne, B. (1982).J. Cell. Biochem. 20149–161.
Seawright, A., Fletcher, J.M., Fantes, J.A., Morrison, H., Porteous, D.J., Li, S.S.L., Hastie, N.D., and van Heyningen, V. (1988).Somat. Cell Mol. Genet. 1421–30.
Feinberg, A., and Vogelstein, B. (1983).Anal. Biochem. 1346–13.
Bartels, I., Grzeschik, K.H., Cooper, D.N., and Schmidtke, J. (1986).Am. J. Hum. Genet. 38280–287.
Scambler, P.J., Wainwright, B.J., Watson, E., Bates, G., Bell, G., Williamson, R., and Farrall, M. (1986).Nucleic Acids Res. 141951–1956.
Cooper, C.S., Park, M., Blair, D.G., Tainsky, M.A., Huebner, K., Croce, C.M., and vande Woude, G.F. (1984).Nature 31129–33.
Collins, M.K.L., Kissonerghis, A.M., Dunne, M.J., Watson, C.J., Rigby, P.W.J., and Owen, M.J. (1985).EMBO J. 41211–1215.
Shafit-Zagardo, B., Maio, J.J., and Brown, F.L. (1982).Nucleic Acids Res. 103175–3193.
Morten, J.E.N., Hirst, M.C., and Porteous, D.J. (1987).Anticancer Res. 7573–588.
Howell, N. (1982).Cytogenet. Cell Genet. 34215–229.
Porteous, D.J., Wilkinson, M.W., Fletcher, J.M., and van Heyningen, V. (1989).Genomics 5680–684.
Spence, M.A., and Tsui, L.C. (1987).Cytogenet. Cell Genet. 46170–187.
Bickmore, W.A., Maule, J.C., van Heyningen, V., and Porteous, D.J. (1989).Somat. Cell Mol. Genet. 15229–235.
Pritchard, C., and Goodfellow, P.N. (1986).EMBO J. 5979–985.
Pritchard, C.A., and Goodfellow, P.N. (1987).Genes Dev. 1172–178.
Xu, W., Gorman, P.A., Rider, S.H., Hedge, P.J., Moore, G., Pritchard, C., Sheer, D., and Solomon, E. (1988).Proc. Natl. Acad. Sci. U.S.A. 858563–8567.
Goss, S.J., and Harris, H. (1975).Nature 255680–684.
Cox, D.R., Pritchard, C.A., Uglum, E., Casher, D., Kobori, J., and Myers, R.M. (1989).Genomics 4397–407.
Benham, F., Hart, K., Crolla, J., Bobrow, M., Francavilla, M., and Goodfellow, P.N. (1989).Genomics 4509–517.
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Porteous, D.J., Dorin, J.R., Wilkinson, M.M. et al. SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region. Somat Cell Mol Genet 16, 29–38 (1990). https://doi.org/10.1007/BF01650477
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DOI: https://doi.org/10.1007/BF01650477