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Molecular cloning and localization to chromosome 6 of mouseINT1L1 gene

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Somatic Cell and Molecular Genetics

Abstract

The human INT1L1 gene, which exhibits homology to the protooncogene INT1 is very closely linked to the MET gene and cystic fibrosis locus on human chromosome 7. In the present study we have isolated overlapping genomic clones that correspond to the mouse homolog of the INT1L1 gene and have used the cloned DNA as probes to examine the distribution of the mouse INT1L1 gene within a series of 35 mouse-hamster somatic cell hybrids. These analyses have localized the INT1L1 gene to mouse chromosome 6. In addition, we demonstrate that the mouse INT1L1 and MET genes are coamplified in lines of spontaneously transformed mouse NIH3T3 cells, indicating that these genes may remain closely linked within the mouse genome.

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Authors and Affiliations

  1. Chemical Carcinogenesis Section, Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, SWS 6JB, London, UK

    A. M. -L. Chan, P. J. Mitchell & C. S. Cooper

  2. Department of Tumour Biology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Plesmanlaan 121, 1066 CX, The Netherlands

    J. Hilkens & V. Kroezen

  3. Department of Biochemistry, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK

    P. Scambler, B. J. Wainwright & R. Williamson

Authors
  1. A. M. -L. Chan
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  2. J. Hilkens
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  3. V. Kroezen
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  4. P. J. Mitchell
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  5. P. Scambler
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  6. B. J. Wainwright
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  7. R. Williamson
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  8. C. S. Cooper
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Chan, A.M.L., Hilkens, J., Kroezen, V. et al. Molecular cloning and localization to chromosome 6 of mouseINT1L1 gene. Somat Cell Mol Genet 15, 555–562 (1989). https://doi.org/10.1007/BF01534916

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  • DOI: https://doi.org/10.1007/BF01534916

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