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Sequence homology and structure predictions of the creatine kinase isoenzymes

  • Molecular Biology of Creatine Kinases
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Abstract

Comparisons of the protein sequences and gene structures of the known creatine kinase isoenzymes and other guanidino kinases revealed high homology and were used to determine the evolutionary relationships of the various guamidino kinases. A ‘CK framework’ is defined, consisting of the most conserved sequence blocks, and ‘diagnostic boxes’ are identified which are characteristic for anyone creatine kinase isoenzyme (e.g. for vertebrate B-CK) and which may serve to distinguish this isoenzyme from all others (e.g. from M-CKs and Mi-CKs). Comparison of the guanidino kinases by near-UV and far-UV circular dichroism further indicates pronounced conservation of secondary structure as well as of aromatic amino acids that are involved in catalysis.

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Abbreviations

GuaK:

guanidino kinase

CK:

creatine kinase

B-and M-CK:

brain and muscle cytosolic CK isoenzyme

Mi-CK:

mitochondrial CK isoenzyme

ArgK:

arginine kinase

Cr:

creatine

PCr:

phosphorylcreatine

PArg:

phosphorylarginine

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Authors and Affiliations

  1. Institute for Cell Biology, ETH Hönggerberg, CH-8093, Zürich, Switzerland

    S. M. Mühlebach, M. Gross, T. Wirz, T. Wallimann & J. -C. Perriard

  2. Department of Transplant Surgery, Research Division, University Hospital, Anichstr. 35, A-6020, Innsbruck, Austria

    M. Wyss

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  1. S. M. Mühlebach
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  2. M. Gross
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  3. T. Wirz
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  4. T. Wallimann
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  5. J. -C. Perriard
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  6. M. Wyss
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Mühlebach, S.M., Gross, M., Wirz, T. et al. Sequence homology and structure predictions of the creatine kinase isoenzymes. Mol Cell Biochem 133, 245–262 (1994). https://doi.org/10.1007/BF01267958

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