Abstract
A simple chromogenic staining procedure is described for the specific identification of biliverdin reductase (BLVR) after gel electrophoresis. Using this procedure a gene for BLVR was assigned to the p14→cen region of human chromosome 7 in human-mouse somatic cell hybrids. Incidentally, the study indicated that the NADH- as well as the NADPH-dependent BLVR activities are due to one and the same enzyme and that, most probably, only one gene in the human genome codes for BLVR and the BLVR is a monomer in its functional configuration.
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This study was performed within the framework of Project 5A12, Genetical Studies on Human Enzymes, of the Medical Faculty, State University of Leiden, and was aided in part by the Foundation for Medical Research FUNGO (Project 13-23-17) which was subsidized by the Netherlands Organization for Advancement of Pure Research (ZWO) and by the Deutsche-Forschungs gemeinschaft (Gr. 373/11).
A part of this work was presented at the Sixth International Conference on Human Gene Mapping, Oslo, Norway, June 1981, and the relevant abstract will appear in its proceedings (Cytogenet. Cell Genet. 32, 1982).
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Meera Khan, P., Wijnen, L.M.M., Wijnen, J.T. et al. Electrophoretic characterization and genetics of human biliverdin reductase (BLVR; EC 1.3.1.24); assignment of BLVR to the p14 → cen region of human chromosome 7 in mouse-human somatic cell hybrids. Biochem Genet 21, 123–133 (1983). https://doi.org/10.1007/BF02395396
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DOI: https://doi.org/10.1007/BF02395396