Summary
Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.
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References
Viola, R. & H.V. Davies, 1992. A microplate reader assay for rapid enzymatic quantification of sugars in potato tubers.Potato Research 35: 55–58.
Dixon, M. & E.C. Webb, 1979. Enzymes. Academic Press, New York.
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Mitchell, S., Dobson, P., Davies, H.V. et al. Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay. Potato Res 41, 383–386 (1998). https://doi.org/10.1007/BF02358970
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DOI: https://doi.org/10.1007/BF02358970