Abstract
Callus cultures fron non-organogenic, young and one-year old, and morphogenic calli were used to assess the value of isozymes analysis for the prediction of morphogenic capacity by studying esterase, peroxidase and acid phosphatase. Basic isozyme patterns of each enzyme for the callus were retained in all the callus stages and in the callus which has differentiated into shoots. With the development of shoot and/or root some conspicuous isozymes appeared for esterase and acid phosphatase and some disappeared for peroxidase. As the isozyme changes became apparent only after shoot or root initiation these enzymes could not be used as markers to distinguish between morphogenic and non-morphogenic calli.
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Chawla, H.S. Isozyme modifications during morphogenesis of callus from barley and wheat. Plant Cell Tiss Organ Cult 12, 299–304 (1988). https://doi.org/10.1007/BF00034372
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DOI: https://doi.org/10.1007/BF00034372