Abstract
KNO3 concentration was found to significantly affect the anther culture of wheat (Triticum aestivum L.). When KNO3 was increased from 0 to 15 mM (in cultivar Jinghua 1) or from 10 to 15 mM (in cultivars 2531-10, Xiaoyan 759 and Norin 10), the callus induction frequency increased significantly. When KNO3 was increased further above 20 mM, the callus induction frequency decreased significantly in all the tested cultivars. The subsequent frequency of green plantlet regeneration increased significantly, and the ratio of green to albino regenerants increased sharply when KNO3 concentration increased. Further experiments found that the decrease of callus induction frequency in the medium with too much KNO3 might be caused by NO3 - ion alone, while the effect of KNO3 on green plantlet regeneration might be caused by both K+ and NO3 - ions, and that the effects of NO3 - concentration were independent of NH4 + concentration in the medium.
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Feng, G.H., Ouyang, J. The effects of KNO3 concentration in callus induction medium for wheat anther culture. Plant Cell Tiss Organ Cult 12, 3–12 (1988). https://doi.org/10.1007/BF00043102
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DOI: https://doi.org/10.1007/BF00043102