Abstract
In a field cropped with wheat, a high and low level of soil conduciveness to take-all were induced by applying a nitrogen fertilizer with either calcium nitrate or ammonium sulphate. From these two soils, two representative populations of fluorescent pseudomonads were tested for their in situ behaviour. Take-all index and root dry weight were assessed on plants cropped in soils infested with Gaeumannomyces graminis var tritici (Ggt) and each bacterized with one of the isolates of fluorescent pseudomonads. The bacteria tested can be split into three groups: antagonists which reduce take-all, deleterious isolates which aggravate the disease and neutral without evident effect on the disease. The predominance of antagonistic fluorescent pseudomonads in the NH4-treated soil and the predominance of deleterious ones in the NO3-treated soil was confirmed after statistical analysis. The microbial impact on take-all must be more considered as the resulting effect of divergent activities of both rhizobacteria types than the only consequences of the presence of antagonistic pseudomonads. All the high cyanogenic pseudomonads were antagonists in situ and were more numerous in the NH4-treated soil than in the NO3-treated soil.
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References
Ahl, P, Voisard, C and Defago, G 1986 Iron boundsiderophores, cyanic acid, and antibiotics involved in suppression of Thielaviopsis basicola by a Pseudomonas fluorescens strain. J. Phytopathol. 116, 121–134.
Aström, B and Gerhardson, B 1989 Wheat cultivar reactions to deleterious rhizosphere bacteria under gnobiotic conditions. Plant and Soil 117, 157–165.
Bakker, A W and Schippers, B 1987 Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp.-mediated plant growth-stimulation. Soil Biol. Biochem. 19, 451–457.
Brown, M E and Hornby, D 1987 Effects of nitrate and ammonium on wheat roots in gnobiotic culture: Amino acids, cortical cell and death and take-all (caused by Gaeumannomyces graminis var, tritici). Soil Biol. Biochem. 19, 567–573.
Castric, K F and Castric, P A 1983 Method for rapid detection of cyanogenic bacteria. Appl. Environ. Microbiol. 45, 701–702.
Curl, E A and Truelove, B 1986 The Rhizosphere. Springer-Verlag, Berlin 288 p.
Defago G, Berling C H, Burger U, Haas D, Kahr G, Keel C, Voisard C, Whirthner Ph and Wütrich B 1990 Suppression of black root rot of tobacco by a Pseudomonas strain: Potential application and mechanism. In Biological Control of Soil-borne Plant Pathogens. Ed. D Hornby. pp 93–103. CAB Interantional.
Elliott L F and Lynch J M 1985 Plant growth-inhibitory pseudomonads colonizing winter wheat (Triticum aestivum L.) roots. Plant and Soil 57–65.
Fahy, P C and Lloyd, A B 1983 The fluorescent Pseudomonas. In Plant Bacterial Disease: A Diagnostic Guide. Eds. P C Fahy and G SPersley p 145. Academic Press. London.
Feigl, F and Anger, V 1966 Replacement of benzidine by copper ethylacetoacetate and tetra base as spot-test reagent for hydrogen cyanide and cyanogen. Analyst 91, 282–284.
Foucart T 1985 Programmation sur Micro-ordinateurs. Masson, F-75280 Paris cedex 06. 243 p.
Howell, C R and Stipanovic, R D 1979 Control of Rhizoctonia solani on cotton seedling with Pseudomonas fluorescents and with an antibiotic produced by a bacterium. Phytopathology 69, 480–482.
Huber, D M 1969 Effect of nitrogen fertilization on take-all of winter wheat. Phytopathology 59, 12.
Huber, D M 1981 The role of nutrients and chemicals. In Biology and Control of Take-all. Eds. M J CAsher and P J Shipton. pp 317–341. Academic Press, London.
Kanner, D, Gerber, N and Bartha, R 1978 Pattern of phenazine pigment production by a strain of Pseudomonas aeruginosa. J. Bacteriol. 134, 690–692.
Lambers, H 1982 Cyanide-resistant respiration: A non-phophorylating electron transport pathway acting as an energy overflow. Physiol. Plant 55, 478–485.
Laties, G 1982 The cyanide-resistant, atlernative path in higher plant respiration. Annu. Rev. Plant Physiol. 33, 519–555.
Legget M and Sivasithamparam K 1986 Interaction of fluorescent pseudomonads, hyphae of the take-all fungus and growth of wehat roots in soil. In Proceedings of the 1986 British Crop Protection Conference-Pests and Diseases, pp 1177–1184.
Lucas, P, Sarniguet, A, Collet, J M and Lucas, M 1989 Réceptivité des sols au piétin échaudage (Gaeumannomyces graminis var. tritici): Influence de certaines techniques culturales. Soil Biol. Biochem. 21, 1073–1078.
McNish, G C and Speijers, J 1982 The use of ammonium fertilizers to reduce the severity of take-all (Gaeumannomyces graminis var tritici) on wheat in western Australia. Ann. Appl. Biol. 100, 83–90.
Nagashima, S and Osawa, T 1981 Spectrophotometric determination of cyanide with isonicotinic acid and barbituric acid. Int. J. Environ. Anal. Chem. 10, 99–106.
Palleroni, N J 1984 Pseudomonas. In R EBuchanan and N E Gibbons. Bergey's Manual of Systematic Bacteriology. Eds. Williams, Wibinsends Vol. 1, pp 141–199.
Sarniguet, A, Lucas, P and Lucas, M 1992 Relationship between take-all development in the field, soil conduciveness to the disease and populations of fluorescent pseudomonads and nitrogen fertilizers. Plant and Soil 145, 17–27.
Sands, D C and Rovira, A D 1970 Isolation of fluorescent pseudomonads with a selective medium. Appl. Microbiol. 20, 513–514.
Smiley, R W and Cook, R J 1973 Relationship between take-all of wheat and rhizosphere pH in soils fertilized with ammonium vs. nitrate-nitrogen. Phytopathology 63, 882–890.
Smiley, R W 1978a Antagonists of Gaeumannomyces graminis from the rhizoplane of wheat in soils fertilized with ammonium- or nitrate-nitrogen. Soil Biol. Biochem. 10, 169–174.
Smiley, R W 1978b Colonization of wheat roots by Gaeumannomyces graminis inhibited by specific soils, microorganisms and ammonium nitrogen. Soil Biol. Biochem. 10, 175–179.
Thomashow, L S and Weller, D M 1988 Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J. Bacteriol. 170, 3499–3508.
Voisard, C, Keel, C, Haas, D and Defago, G 1989 Cyanide production by Pseudomonas fluorescens helps suppress black root rot of tobacco under gnotobiotic conditions. EMBO J. 8, 351–358.
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Sarniguet, A., Lucas, P., Lucas, M. et al. Soil conduciveness to take-all of wheat: Influence of the nitrogen fertilizers on the structure of populations of fluorescent pseudomonads. Plant Soil 145, 29–36 (1992). https://doi.org/10.1007/BF00009538
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DOI: https://doi.org/10.1007/BF00009538