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Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): a role in hormone-mediated cell division?

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Abstract

Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medicago sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows homology to the β-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves, and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.

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Authors and Affiliations

  1. Institut des Sciences Végétales, 91198, Gif-sur-Yvette, France

    Heather I. McKhann, Florian Frugier, György Petrovics, Teodoro Coba de la Peña, Edouard Jurkevitch, Spencer Brown, Eva Kondorosi, Adam Kondorosi & Martin Crespi

  2. Institute of Genetics, Biological Reseach Center, 6701, Szeged, Hungary

    Adam Kondorosi

  3. Molecular Cell Biology, Utrecht University, the Netherlands

    Heather I. McKhann

  4. Laboratory of Cellular Oncology, NCI, National Institutes of Health, Bethesda, MD, 20892, USA

    György Petrovics

  5. Departamento de Recursos Naturais e Medio Ambiente, Universidade de Vigo, Apdo 874, 36200, Vigo, Spain

    Teodoro Coba de la Peña

  6. Department of Microbiology, Faculty of Agriculture, 76100, Rehovot, Israel

    Edouard Jurkevitch

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  1. Heather I. McKhann
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  2. Florian Frugier
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  3. György Petrovics
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  4. Teodoro Coba de la Peña
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  5. Edouard Jurkevitch
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  6. Spencer Brown
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  7. Eva Kondorosi
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  8. Adam Kondorosi
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  9. Martin Crespi
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McKhann, H.I., Frugier, F., Petrovics, G. et al. Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): a role in hormone-mediated cell division?. Plant Mol Biol 34, 771–780 (1997). https://doi.org/10.1023/A:1005899410389

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  • DOI: https://doi.org/10.1023/A:1005899410389

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