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Sequencing and characterization of the kinesin-related geneskatB andkatC ofArabidopsis thaliana

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Abstract

Complementary DNAs of two kinesin-related genes,katB andkatC, were isolated fromArabidopsis thaliana and sequenced. The carboxyl-terminal regions of the polypeptides encoded by these genes, especially the presumptive ATP-binding and microtubule-binding domains, share significant sequence homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structures of KatB and KatC proteins include a large globular domain in the carboxyl-terminal region and a small globular domain in the amino-terminal region that are separated by a long α-helical coiled-coil with heptad repeats. A truncated KatC polypeptide (KatC(207–754)), which includes the carboxylterminal region of KatC, was expressed inEscherichia coli and was shown to possess microtubule-stimulated ATPase activity and to bind to microtubules in an ATP-sensitive manner, both of which are characteristics of kinesin and kinesin-like proteins.

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Authors and Affiliations

  1. Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Hisayuki Mitsui, Kimihiko Nakatani & Hideo Takahashi

  2. Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Japan

    Kazuko Yamaguchi-Shinozaki & Kazuo Shinozaki

  3. Protein Engineering Research Institute, Suita, Osaka, Japan

    Ken Nishikawa

Authors
  1. Hisayuki Mitsui
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  2. Kimihiko Nakatani
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  3. Kazuko Yamaguchi-Shinozaki
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  4. Kazuo Shinozaki
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  5. Ken Nishikawa
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  6. Hideo Takahashi
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Mitsui, H., Nakatani, K., Yamaguchi-Shinozaki, K. et al. Sequencing and characterization of the kinesin-related geneskatB andkatC ofArabidopsis thaliana . Plant Mol Biol 25, 865–876 (1994). https://doi.org/10.1007/BF00028881

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  • DOI: https://doi.org/10.1007/BF00028881

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