Abstract
The maize inbred line A188 is popularly used for the production of embryogenic cell lines. A188, maintained at the University of Minnesota, was found upon molecular analysis to contain 2 to 4 copies of a DNA sequence very similar in structure to transposable Mu1 elements, which have been implicated in Robertson's Mutator system. These Mu1-like elements are in the same chromosomal locations in sibling plants and in A188 cell cultures derived from them. This suggests that the elements are in an inactive state and do not undergo transposition. However, we have observed that they are not modified at the target sites for certain restriction endonucleases. Possible causes for the apparent lack of transposition of these Mu1-like elements in these A188 lines are discussed. Inasmuch as the elements do not transpose, they must be maintained in this line as homozygous Mendelian elements by self-pollination.
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References
Alleman M, Freeling M: The Mu transposable elements of maize: evidence for transposition and copy number regulation during development. Genetics 112: 107 (1986).
Armstrong CL, Green CE: Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 163: 207 (1985).
Barker F, Thompson DV, Talbot DR, Swanson J, Bennetzen JL: Nucleotide sequence of the maize transposable element Mu1. Nucleic Acid Res 12: 5955 (1984).
Bennetzen JL: Transposable element Mu1 is found in multiple copies only in Robertson's Mutator maize lines. J Mol Appl Genet 2: 519 (1984).
Bennetzen JL, Swanson J, Taylor WC, Freeling M: DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci USA 18: 4125 (1984).
Benzion G, Philips RL, Rines HW: Case histories of genetic variability in vitro. In: Vasil IK (ed.) Plant Regeneration and Genetic Variability. Academic Press, Orlando, Florida (1986) in press.
Chandler V, Rivin C, Walbot V: Stable nonMutator stocks of maize have sequences homologous to the Mu1 transposable element. Genetics 114: 1007 (1986).
Chandler V, Walbot V: DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci USA 83: 1767 (1986).
Dawid IB: DNA-DNA hybridization on membrane filters: a convenient method using formamide. Biochim Biophys Acta 477: 191 (1977).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: version 11. Plant Molec Biol Rep 1: 19 (1983).
Federoff NV: Notes on cloning maize DNA. Maize Genet Coop Newsl 57: 154 (1983).
Freeling M: Plant transposable elements and insertion sequences. Annual Rev Plant Physiol 35: 277 (1984).
Freeling M, Taylor WC, Bennetzen JL: Element Mu1. Maize Genet Coop Newsl 57: 12 (1983).
Gingeras TA, Greenough L, Schildkraut I, Roberts RJ: Nucleic Acids Res 9: 4525 (1981).
Gorman MB, Peterson PA: The interaction of controlling element components in a tissue culture system. Maydica 23: 173 (1978).
Kidwell MG: Hybrid dysgenesis in Drosophila melanogaster: the relationship between the P-M and the I-R interaction systems. Genet Res 33: 205 (1979).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982).
Rigby PWJ, Dieckmann M, Rhodes C, Berg P: Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 237 (1977).
Robertson DS: Characterization of a mutator system in maize. Mutat Res 51: 21 (1978).
Robertson DS, Stinard PS, Wheeler JG, Morris DW: Genetic and molecular studies on germinal and somatic instability in Mutator-induced aleurone mutants of maize. In: Freeling M (ed.) Plant Genetics. Alan R. Liss Inc. (1985) p. 317.
Sheridan WF: Growth of corn cells in culture. J Cell Biol 67: 396a (abstract) (1975).
Sheridan WF: Black Mexican Sweetcorn: its use for tissue culture. In: Sheridan WF (ed.) Maize for Biological Research. Plant Molecular Biology Assoc., University of N. Dakota (1982) p. 385.
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 502 (1975).
Strommer JN, Hake S, Bennetzen J, Taylor WC, Freeling M: Regulatory mutants of the maize Adh1 gene caused by DNA insertions. Nature 300: 542 (1982).
Walbot V, Chandler V, Taylor L: Alterations in the mutator transposable element family of Zea mays. In: Freeling M (ed.) Plant Genetics. Alan R. Liss Inc. (1985) p. 333.
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Journal paper no. J-12269 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa 50011. Project 2707.
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Masterson, R.V., Biagi, K., Wheeler, J.G. et al. An embryogenic cell line of maize from A188 (Minnesota) contains Mu1-like elements. Plant Mol Biol 10, 273–279 (1988). https://doi.org/10.1007/BF00027404
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DOI: https://doi.org/10.1007/BF00027404