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A simple plant polytene chromosome system, and its use for in situ hybridisation

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Abstract

When the cotyledons of Pisum sativum are cultured in the presence of 2–4D, large populations of cells with polytene chromosomes are stimulated to enter prophase; the use of these chromosome for studies of in situ hybridisation with nucleic acids is described.

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, England

    David Roy Davies & Christopher Ashley Cullis

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  1. David Roy Davies
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  2. Christopher Ashley Cullis
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Davies, D.R., Cullis, C.A. A simple plant polytene chromosome system, and its use for in situ hybridisation. Plant Mol Biol 1, 301–304 (1982). https://doi.org/10.1007/BF00027561

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  • DOI: https://doi.org/10.1007/BF00027561

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