Abstract
The pathogenicity of a strain of Conidiobolus coronatus isolated from Aenolamia postica was investigated in 152 mice, 42 guinea pigs and 47 hamsters. Four routes of inoculation were used: intraperitoneal, subcutaneous, intratracheal and scarification in the oral mucosa. Each animal was inoculated 3 times at 3 week intervals. Animals were sacrificed at 1, 8, 15, 45 of 225 days after the first inoculation. Observations of macroscopic lesions (nodules, abscesses, adhesions, etc.) direct examinations, cultures and histopathological studies of different tissues were performed. The aforementioned lesions of tissue were the most frequently found and all of them were resolved by spontaneous cure. The animals inoculated intraperitoneally and subcutaneouslly presented the highest number of positive results. The histopathological features at the 1st and 8th days after inoculation showed an acute inflamatory infiltrate with well preserved fungus filaments; at the 15th day granulomas with giant cells were observed containing fragmented and disintegrated fungal hyphae; at the 45th and 225th days a fibrosis reaction was observed and no fungal filaments, could be found. In no animal was rhinoentomophthoromycosis never reproduced, and according to these results it is considered that either this strain is not a pathogen or its pathogenicity is very low.
Resumen
A partir de una cepa de Conidiobolus coronatus aislada de Aenolamia postica, se inocularon 152 ratones, 42 cobayos y 47 hamsters por vías intraperitoneal (IP), subcutánea (SC), intratraqueal y por escarificación en la mucosa oral; cada animal se inoculó 3 veces a intervalos de una semana. Los animales se sacrificaron a los 1, 8, 15, 45 y 225 días de la primera inoculación, haciéndose estudios de alteraciones macroscópicas (nódulos, abscesos, adherencias), exámenes directos, cultivos e histopatología. Las alteraciones macroscópicas de los tejidos fueron las más frecuentes y todas evolucionaron a la curación espontánea. Los animales presentaron un mayor múmero de estudios positivos cuando se inocularon por vía IP y SC. Los estudios histopatológicos hechos al 1° y 8° día, demostraron un infiltrado inflamatorio agudo y filamentos fúngicos bien conservados; a los 15 días se observaron granulomas con células gigantes y filamentos fragmentados en desintegración; a los 45 y 225 días se observó una reacción de fibrosis sin filamentos fúngicos. Se consideró que en ningún caso se logró reproducir la rinoentomoftoromicosis y de acuerdo a éstos resultados se estima que esta cepa no tiene patogenicidad o bien ésta es muy baja.
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Referencias
Andrade, Z.A.; Araujo, L.P., Sherlock, I.A. & Cheever, E.A. (1967). Nasal Granuloma caused by Entomophthora coronata. The American Journal of Tropical Medicine and Hygiene. 16: 31–39.
Bras, G., Gordon, C.C., Emmons, C.W., Prendegast, K.M. & Sugar, M. (1965). A case of phycomycosis observed in Jamaica infection with Entomophthora coronata. Journal of Tropical Medicine and Hygiene. 14: 141–145.
Clark, B.M. (1967). The epidemiology of phycomycosis. Systemic mycoses. A Ciba Foundation Symposium. Little Brown and Company. Boston. pp. 179–192.
Destombes, P., Poirier, P. & Nazimoff, O. (1970). Mycoses profondes reconnues en 9 ans de pratique histopathologique a l'Institut Pasteur du Cameroun. Bulletin de la Société de Pathologie Exotique. 63: 310–315.
Destombes, P. & Ravisse, P. (1976). Les granulomes inflamatoires d'origine fongique. Annales d' Anatomie pathologique, Paris. 21: 99–116.
Emmons, C.W. & Bridges, C.H. (1961). Entomophthora coronata, the etiologic agent of a Phycomycosis of horses. Mycologia. 53: 307–316.
Fromentin, H. (1975). Résultats préliminaires sur les protéases d'Entomophthora coronata pathogène de mammifères. Bulletin de la Société française de Mycologie Medicale. 4: 215–220.
Fromentin, H. (1976). Activité caséinolytique d'Entomophthora coronata au cours de sa croissance en culture stable. Mycopathologia. 59: 43–45.
Fromentin, H. (1976). Infection experimentale de la souris par des Entomophthorales. Bulletin de la Société Française de Mycologie Médicale. 5: 157–160.
Garrison, R.G., Mariat, F., Boyd, K.S. & Tally, J.F. (1975). Ultrastructural and electron Cytochemical studies of Entomophthora coronata, Annales de Microbiologie.
Gilbert, E.F., Khoury, G.H. & Pore, R.S. (1970). Histopathological identification of Entomophthora phycomycosis. Archives of Pathology. 90: 583–587.
Gustafsson, M. (1965). On the species of the genus Entomophthora Fres. In Sweden. I. Classification and Distribution. Lantbrukshogskolans Annaler. 31: 103–128.
Gustafsson, M. (1965). On species of the genus Entomophthora Fres. in Sweden. II. Cultivation and Physiology. Lantbrukshogslans Annaler. 31: 405–457.
Guzmán, G. & Alcocer-Gómez, L. (1972). Un hongo de importancia biológica en México. Boletín de la Sociedad Mexicana de Micología. 6: 5–8.
Johnston, M.J., Soerensen, B., Saliba, A.M., Lacaz, da S.C., Neto, J.B. & Cruz, J.M. (1967). Ficomicose em muar. Isolamento de Entomophthora coronata. Arquivos do Instituto Biológico. S. Paulo. 34: 51–58.
Jonsson, A.G. (1968). Protease production by species of Entomophthora. Applied. Microbiology. 16: 450–457.
King, D.S. (1976). Systematics of Conidiobolus (Entomophthorales) using numerical taxonomy. II. Taxonomic considerations. Canadian Journal of Botany. 54: 1285–1296.
King, D.S. (1977). Systematics of Conidiobolus (Entomophthorales) using numerical taxonomy. III. Descriptions of recognized species. Canadian Journal of Botany. 55: 718–729.
Lambiase, J.T. & Yendol, W.G. (1977). The fine structure of Entomophthora apiculata its penetration of Trichoplusia ni. Canadian Journal of Microbiology. 23: 452–464.
Martinson, F.D. & Clark, B.M. (1967). Rhinophycomycosis entomophthorae in Nigeria. The American Journal of Tropical Medicine and Hygiene. 16: 40–47.
Ravisse, P., Destombes, P. & Le Gonidec, G. (1976). Dix novelles observations de mycose par Entomophthorales au Cameroun. Bulletin de la Société de Pathologie Exotique. 69: 33–40.
Restrepo, A.M., Greer, D.L., Robledo, M.V., Díaz, G., López, R.M. & Bravo, C.R. (1967). Subcutaneous phycomycosis: Report of the first case observed in Columbia, South America. The American Journal of Tropical Medicine and Hygiene. 16: 34–48.
Restrepo, L.F., Morales, L.F., Robledo, M., Restrepo, A.M. & Restrepo, G. (1973). Rinoficomicosis por Entomophthora coronata en equinos. Antioquia Médica. Columbia. 23: 13–25.
Roy, A.D. & Cameron, H.E. (1972). Rhinophycomycosis entomophthorae occurring in a chimpanzee in the wild in East Africa. The American Journal of Tropical Medicine and Hygiene. 21: 234–237.
Soper, R.S. & Bryan, T.A. (1974). Mammalian Safety of the Aphid-Attacking Fungus Entomophthora nr. thaxteriana. Entomology. 3: 346–347.
Tyrrel, D. & Simpson, J.E. (1975). Glycolytic enzymes in resting spores and vegetative mycelia of Entomophthora pyriformis. Canadian Journal of Microbiology. 21: 301–304.
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López-Martínez, R., Toriello, C., Mier, T. et al. Estudio de la patogenicidad de conidiobolus Coronatus en animales de experimentacion. Mycopathologia 66, 59–65 (1978). https://doi.org/10.1007/BF00429594
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DOI: https://doi.org/10.1007/BF00429594