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Partial purification and characterization of the endotoxin from Aspergillus fumigatus

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Summary

The endotoxin fromAspergillus fumigatus was studied and appears to be protein in character. Attempts to purify the material by fractional precipitation with acetone resulted in increase of hemolytic and toxic potencies. The hemolytic activity of the toxin is stable from pH 3.6 to 8.4.

Electrophoretic analysis enabled the assignment of hemolytic activity to a definite electrophoretic component.

Chromatography with DEAE cellulose using gradient elution effected increase in hemolytic activity. The bioassay for toxic activity is less quantitative than the assay for the hemolytic activity.

Column electrophoresis confirmed the assignment of hemolytic potency to an electrophoretic component. Recovery of activity was low in this method.

Both the hemolytic and toxic activities occur in the same electrophoretic component; however, there is evidence that they may be separate components.

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Authors and Affiliations

  1. From the Dept. of Biochem., Northwestern Univ. Med. School, Chicago 11, Ill.

    Evelyn M. Rau, Evelyn B. Tilden & Virgil L. Koenig

  2. The Brookfield Zoo, Brookfield, Ill.

    Evelyn M. Rau, Evelyn B. Tilden & Virgil L. Koenig

Authors
  1. Evelyn M. Rau
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  2. Evelyn B. Tilden
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  3. Virgil L. Koenig
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Additional information

Adapted from a Thesis submitted byEvelyn M. Rau to Northwestern University in partial fulfillment of the M. S. degree in Biochemistry, June, 1960.

Supported by Grant E-2435 from the National Institute of Allergy and Infectious Diseases.

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Rau, E.M., Tilden, E.B. & Koenig, V.L. Partial purification and characterization of the endotoxin from Aspergillus fumigatus. Mycopathologia et Mycologia Applicata 14, 347–358 (1961). https://doi.org/10.1007/BF02051549

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  • DOI: https://doi.org/10.1007/BF02051549

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