References
Fristrom, J. W., W. R. Logan, and C. Murphy. 1973. The synthetic and minimal culture requirements for evagination of imaginal discs ofDrosophila melanogaster in vitro. Dev. Biol. 33: 441–456.
Robb, James. 1969. Maintenance of imaginal discs ofDrosophila melanogaster in chemically defined media. J. Cell Biol. 41: 876–884.
Ephrussi, B., and G. W. Beadle. 1936. A technique of transplantation inDrosophila. Am. Nat. 70: 218–225.
Zweidler, A., and L. H. Cohen. 1971. Large-scale isolation and fractionation of organs ofDrosophila melanogaster larvae. J. Cell Biol. 51: 240–248.
Eugene, O. M., and J. W. Fristrom. 1978. The mass isolation of imaginal discs. In: M. Ashburner, and T. R. F. Wright (Eds.),The Genetics and Biology of Drosophila. Academic Press, New York, pp. 121–126.
Fristrom, J. W. 1972. The biochemistry of imaginal disc development. In: H. Ursprung, and R. Nothiger (Eds.),The Biology of Imaginal Discs. Results and Problems in Cell Differentiation. Vol. 5. Springer-Verlag, Berlin and New York, pp. 109–154.
Chihara, C., W. Petri, J. W. Fristrom, and D. King. 1972. The assay of ecdysones and juvenile hormones onDrosophila imaginal discsin vitro. J. Insect Physiol. 18: 1115–1123.
Benjaminson, M. A. 1969. Conjugates of chitinase with fluorescein isothiocyanate or lisoamine rhodamine as specific stains for chitinin situ. Stain Technol. 44: 27–31.
Fristrom, J. W., R. J. Hill, and F. Watt. 1978. The procuticle ofDrosophila: Heterogeneity of urea-soluble proteins. Biochemistry 17: 3917–3924.
Fristrom, J. W., and H. K. Mitchell. 1965. The preparative isolation of imaginal discs from larvae ofDrosophila melanogaster. J. Cell Biol. 27: 445–448.
Nishiura, J. T., and J. W. Fristrom. 1975. Effect of insect hormones on RNA polymerases of mass-isolated imaginal discs ofDrosophila melanogaster culturedin vitro. Proc. Nat. Acad. Sci. U.S.A. 72: 2984–2988.
Yund, M. A., D. S. King, and J. W. Fristrom. 1978. Ecdysteroid receptors in imaginal discs ofDrosophila melanogaster. Proc. Nat. Acad. Sci. U.S.A. 75: 6039–6043.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eugene, O., Yund, M.A. & Fristrom, J.W. Preparative isolation and short-term organ culture of imaginal discs ofDrosophila melanogaster . Tca Manual 5, 1055–1062 (1979). https://doi.org/10.1007/BF00919788
Issue Date:
DOI: https://doi.org/10.1007/BF00919788