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Peptide synthesis containing a B-cell and a T-cell epitope on dextran beads and evaluation of humoral response against bead-peptide construct

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Abstract

Cross-linked dextran beads were chemically modified withFmoc-βAla to give an amino functionalized support suitablefor solid-phase peptide synthesis. On this support, apeptide comprising B-cell and T-cell epitopes in tandem wassynthesized manually by Fmoc procedure monitoring thecoupling reaction by bromophenol blue procedure andstandard Kaiser test. The quality of peptide synthesis wasverified by RP-HPLC and mass spectrometry. The size andmorphologic characteristics of dextran beads were conservedafter peptide synthesis. Furthermore, we analyzedimmunogenicity in mice of the T-B peptide on dextran beadscompared with T-B peptide immunogenicity when administeredin CFA/IFA. In both cases, titers were high and there wasnot a significant difference in antibody titers betweengroups (p<0.05). But in contrast CFA they are biocompatibleand did not induce any adverse reaction at the site ofinjection. This experiment suggests that the peptidessynthesis on dextran beads can be used to raise theimmunogenicity of synthetic peptide in vaccines ortherapeutics.

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Authors and Affiliations

  1. Divisiones de Química Física;, Centro de Ingeniería Genética y Biotecnología, Apdo 6162, 10600, Ciudad Habana, Cuba

    Luis J. Cruz, Rubén Padrón, Luis J. González, Hilda E. Garay, Viviana Falcón, Eulises Rodríguez & Osvaldo Reyes

  2. Vacunas, Centro de Ingeniería Genética y Biotecnología, Apdo 6162, 10600, Ciudad Habana, Cuba

    Julio C. Aguilar & Enrique Iglesias

Authors
  1. Luis J. Cruz
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  2. Rubén Padrón
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  3. Luis J. González
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  4. Julio C. Aguilar
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  5. Enrique Iglesias
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  6. Hilda E. Garay
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  8. Eulises Rodríguez
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  9. Osvaldo Reyes
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Correspondence to Luis J. Cruz.

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Cruz, L.J., Padrón, R., González, L.J. et al. Peptide synthesis containing a B-cell and a T-cell epitope on dextran beads and evaluation of humoral response against bead-peptide construct. Letters in Peptide Science 7, 229–237 (2000). https://doi.org/10.1023/A:1011201104069

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