Abstract
Lipid extracts of the red algaGracilaria longa were studied by1H- and13C-NMR spectroscopy. Peaks in the13C-NMR spectra attributable to sterols, chlorophylls and carotenoids allowed free and acylated cholesterol, chlorophylla and lutein to be identified as the most abundant components of these classes. A content of 0.5 ± 0.1 μmoles of total cholesterol/g wet alga was estimated from the1H-NMR spectrum, which also allowed the determination of the phosphatidylcholine/total lipid molar ratio (9.5 ± 0.5%). The13C-NMR spectroscopic experiments provided information on the position of the double bonds on the fatty acid residues. A comparison between NMR spectra of lipid extracts obtained for wet and dried alga showed that the alga undergoes both a dramatic peroxidation and some glycolipid degradation during the drying process.
Similar content being viewed by others
References
Armisen R, Galatas F (1987) Production, properties and uses of Agar. In McHugh DJ (ed.), Production and utilization of products from commercial seaweed, F.A.O. Fish. Tech. Pap., Rome, 1–57.
Basti MM, LaPlanche LA (1990)1H-,13C- and31P-NMR studies of dioctanoylphosphatidylcholine and dioctanoylthiophosphatidylcholine. Chem. Phys. Lipids 54: 99–113.
Breitmaier E, Voelter W (1987) Carbon-13 NMR Spectroscopy, VCH Verlagsgesellschaft mbH, Weinheim, Germany, 335–336.
Dembitsky VM, Pechenkina-Shubina EE, Rozentsvet OA (1991) Glycolipids and fatty acids of some seaweeds and marine grasses from the Black Sea. Biochemistry 30: 2279–2283.
Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497–509.
Harman D (1956) A theory based on free radical and radiation chemistry. J. Gerontol. 11: 298–300.
Henriquez P, Trucco R, Silva M, Sammes PG (1971) Cholesterol inIridaea laminarioides andGracilaria verrucosa. Phytochemistry 11: 1171.
May GL, Wright LC, Holmes KT, Williams PG, Smith ICP, Wright PE, Fox RM, Mountford CE (1985) Assignment of methylene proton resonances in NMR spectra of embryonic and transformed cells to plasma membrane triglyceride. J. Biol. Chem. 261: 3048–3053.
Nyberg H (1984) The influence of ionic detergents on the phospholipid fatty acid compositions ofPorphyridium purpureum. Phytochemistry 24: 435–440.
Nyberg H, Koskimies-Soininen K (1983) The glycolipid fatty acids ofPorphyridium purpureum cultured in the presence of detergents. Phytochemistry 23: 751–757.
Pettitt TR, Jones L, Harwood JL (1988) Lipids of the marine red algae,Chondrus crispus andPolysiphonia lanosa. Phytochemistry 28: 399–405.
Pohl P, Zurheide F (1979) Fatty acids and lipids of marine algae and the control of their biosynthesis by environmental factors. In Hoppe HA, Levring T, Tanaka Y (eds), Marine Algae in Pharmaceutical Science, Walter de Gruyter, Berlin, Germany, 473–523.
Pollesello P, Eriksson O, Kvam BJ, Paoletti S, Saris NEL (1991)1H-NMR studies of lipid extracts of rat liver mitochondria. Biochem. Biophys. Res. Comm. 179: 904–911.
Rowan KS (1989) Photosynthetic pigments of algae. Cambridge University Press, Cambridge, UK, 112–113.
Sillerud LO, Han CH, Bitensky MW, Francendese AA (1986) Metabolism and structure of triacylglycerols in rat epididymal fat pad adipocytes determined by13C nuclear magnetic resonance. J. Biol. Chem. 261: 4380–4388.
Son BW (1990) Glycolipids fromGracilaria verrucosa. Phytochemistry 29: 307–309.
Sze DY, Jardetzky O (1990) Characterization of lipid composition in stimulated human lymphocytes by1H-NMR. Biochim. Biophys. Acta 1054: 198–206.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pollesello, P., Toffanin, R., Murano, E. et al. 1H- and13C-NMR spectroscopic studies of lipid extracts of the red algaGracilaria longa . J Appl Phycol 4, 149–155 (1992). https://doi.org/10.1007/BF02442463
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02442463