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Effects of dietary lipids on body composition and liver function in juvenile red drum, Sciaenops ocellatus

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Abstract

This study was conducted to evaluate the effects of different concentrations of dietary lipids on body composition and liver function in juvenile red drum, Sciaenops ocellatus. Diets were formulated to contain 40% crude protein from solvent-extracted menhaden fish meal and 0, 7, 14 or 21% lipid from menhaden fish oil. The basal diet, without supplemental fish oil, contained lipid at 0.4% of dry weight. The diets were fed to groups of 25 juvenile red drum initially averaging 7.3 ± 0.18 g fish−1 in a recirculating culture system for 8 weeks and weight gain was recorded. After an additional 8 weeks, 16 fish from each treatment were sacrificed and the following measurements were recorded: hepatosomatic index (HSI), intraperitoneal fat (IPF) ratio, and liver α-tocopherol, malondialdehyde (MDA) formation, and cytochrome P-4501A activity (measured as 7-ethoxyresorufin O-deethylase (EROD) activity). The activity of alanine and aspartate aminotransferases and concentrations of α-tocopherol also were measured in plasma.

Weight gain was significantly (p<0.05) affected by dietary lipid concentration, with values ranging from 361% of initial weight for fish fed the basal diet to 527% of initial weight for fish fed the diet containing 7% lipid. The HSI and IPF ratio values also were significantly affected by lipid with the lowest values recorded for fish fed the basal diet and the highest values observed in fish fed the diet containing 21% lipid. Increasing dietary lipid significantly increased oxidative stress as reflected in reduced α-tocopherol in liver and plasma and increased MDA formation in the liver, although no overt pathological signs were observed. These findings suggest that lipid concentrations between 7 and 14%, when the diet contains 60 IU vitamin E kg−1, are likely to limit oxidative stress and result in normal physiological responses of red drum.

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Authors and Affiliations

  1. Department of Wildlife and Fisheries Sciences, Texas A&M University System, College Station, TX, 77843, USA

    S.R. Craig & D.M. Gatlin III

  2. Department of Biological Sciences, University of Texas at El Paso, El Paso, TX 79968, USA

    B.S. Washburn

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  1. S.R. Craig
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  3. D.M. Gatlin III
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Craig, S., Washburn, B. & Gatlin, D. Effects of dietary lipids on body composition and liver function in juvenile red drum, Sciaenops ocellatus. Fish Physiology and Biochemistry 21, 249–255 (1999). https://doi.org/10.1023/A:1007843420128

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