Abstract
Protein synthesis was assessed in rainbow trout (Oncorhynchus mykiss) hearts perfused with medium containing 3H phenylalanine. Isolated hearts from fish acclimated to 5° and 15°C were used as the model system, and were perfused at variable test temperatures and pH. Protein synthesis expressed as nmol PHE mg protein−1 h−1 was two fold higher in hearts from fish acclimated to 15°C and tested at 15°C and extracellular pH 7.6 than in hearts from fish acclimated to 5°C and tested at 5°C and extracellular pH 8.0. The prime determinant of the decreased rate of protein synthesis was thermal history. Fish acclimated to 5°C had lower levels of RNA mg protein−1 than fish held at 15°C. There was a direct linear relationship between the rate of protein synthesis in nmol PHE mg protein−1 h−1 and RNA content. RNA activity (nmol PHE μg RNA−1 h−1 remained constant regardless of thermal history or perfusion condition. Elevated pH resulted in only a marginal decrease in protein synthesis. Test temperature had no effect on in vitro rates of protein synthesis.
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Barron, M.G., Tarr, B.D. and Hayton, W.L. 1987. Temperature-dependence of cardiac output and regional blood flow in rainbow trout, Salmo gairdneri Richardson. J. Fish Biol. 31: 735–744.
Burton, K. 1956. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical J. 62: 315–323.
Foster, A.R., Houlihan, D.F., Hall, S.J. and Burren, L.J. 1992. The effects of temperature acclimation on protein synthesis rates and nucleic acid content of juvenile cod (Gadus morhua L). Can. J. Zool. 70: 2095–2102.
Fuller, S.J. and Sugden, P.H. 1988. Acute inhibition of rat heart protein synthesis during in vitro β-adrenergic stimulation or hypoxia. Am. J. Physiol. 255: E537–E547.
Fuller, S.J, Gaitanaki, C.J. and Sugden, P.H. 1989. Effects of increasing extracellular pH on protein synthesis and protein degradation in the perfused working rat heart. Biochem. J. 259: 173–179.
Goolish, E.M. 1987. Cold-acclimation increases the ventricle size of carp, Cyprinus carpio. J. Therm. Biol. 3: 203–205.
Goolish, E.M., Barron, M.G. and Adelman, I.R. 1984. Thermoacclimatory response of nucleic acid and protein content of carp muscle tissue: influence of growth rate and relationship to glycine uptake by scales. Can. J. Zool. 62: 2164–2170.
Graham, M.S. and Farrell, A.P. 1989. The effect of temperature acclimation and adrenaline on the performance of a perfused trout heart. Physiol. Zool. 62: 38–61.
Graham, M.S. and Farrell, A.P. 1990. Myocardial oxygen consumption in trout acclimated to 5°C and 15°C. Physiol. Zool. 63: 536–554.
Houlihan, D.F., Agnisola, C., Lyndon, A.R., Gray, C. and Hamilton, N.M. 1988. Protein synthesis in a fish heart: Responses to increased power output. J. Exp. Biol. 137: 565–587.
Kent, J., Koban, M. and Prosser, C.L. 1988. Cold-acclimation-induced protein hypertrophy in channel catfish and green sunfish. J. Comp. Physiol. B 158: 185–198.
Kiceniuck, J.W. and Jones, D.R. 1977. The oxygen transport system in trout (Salmo gairdneri) during sustained exercise. J. Exp. Biol. 69: 247–260.
Lanctin, H.P., McMorran, L.E. and Driedzic, W.R. 1980. Rates of glucose and lactate oxidation by the perfused isolated trout (Salvelinus fontinalis) heart. Can. J. Zool. 58: 1708–1711.
Lewis, S.E.M., Kelly, F.J. and Goldspink, D.F. 1984. Pre- and post-natal growth and protein turnover in smooth muscle, heart and slow- and fast-twitch muscles of the rat. Biochem. J. 217: 517–526.
Lougha, P.T. and Goldspink, G. 1985. Muscle protein synthesis rates during temperature acclimation in a eurythermal (Cyprinus carpio) and a stenothermal (Salmo gairdneri) species of teleost. J. Exp. Biol. 118: 267–276.
Morgan, H.E., Chua, B.H.L., Fuller, E.O. and Siehl, D. 1980. Regulation of protein synthesis and degradation during in vitro cardiac work. Am. J. Physiol. 238: E431–E442.
Munro, H.N. and Fleck, A. 1966. The determination of nucleic acids. In Methods of Biochemical Analysis. Vol. 14, pp. 113–176. Edited by D. Glick. Wiley, New York.
Pannevis, M.C. and Houlihan, D.F. 1992. The energetic cost of protein synthesis in isolated hepatocytes of rainbow trout (Oncorhynchus mykiss). J. Comp. Physiol. B 162: 393–400.
Preedy, V.R. and Sugden, P.H. 1989. The effects of fasting or hypoxia on rates of protein synthesis in vivo in subcellular fractions of rat heart and gastrocnemius muscle. Biochem. J. 257: 519–527.
Randall, D.J. and Cameron, J.N. 1973. Respiratory control of arterial pH as temperature changes in rainbow trout Salmo gairdneri. Am. J. Physiol. 225: 997–1002.
Sephton, D.H., Bailey, J. and Driedzic, W.R. 1990. Impact of acute temperature transition on enzyme activity levels, oxygen consumption and exogenous fuel utilization in sea raven (Hemitripterus americanus) hearts. J. Comp. Physiol. B 160: 511–518.
Smith, D.M. and Sugden, P.H. 1983. Differential rates of protein synthesis in vitro and RNA contents of rat heart ventricular and atrial muscle. Biochem. J. 214: 497–502.
Sugden, P.H. and Fuller, S.J. 1991. Regulation of protein turnover in skeletal and cardiac muscle. Biochem. J. 273: 21–37.
Tsukuda, H. Lui, B. and Fugii, K. 1985. Pulsation rate and oxygen consumption of isolated hearts of the goldfish, Carassius auratus, acclimated to different temperatures. Comp. Biochem. Physiol. A 82: 281–283.
Watt, P.W., Marshall, P.A., Heap, S.P., Loughna, P.T. and Goldspink, G. 1988. Protein synthesis in tissues of fed and starved carp, acclimated to different temperatures. Fish Physiol. Biochem. 4: 165–173.
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Sephton, D.H., Driedzic, W.R. Low temperature acclimation decreases rates of protein synthesis in rainbow trout (Oncorhynchus mykiss) heart. Fish Physiol Biochem 14, 63–69 (1995). https://doi.org/10.1007/BF00004291
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DOI: https://doi.org/10.1007/BF00004291