Summary
Various DNA fingerprint probes were applied to Carica papaya and other Carica species for both identification and genetic analysis.
Each of the Carica papaya cultivars is characterized by a specific DNA fingerprints pattern. Various Carica species also have specific patterns which distinguish them from one another. Band sharing levels were used to estimate the relatedness between the various Carica species.
Genetic analysis of 11 progeny from a cross between the Carica papaya cultivars 17/82 and 112 suggests that application of DNA fingerprinting to Carica papaya breeding, could make the process more efficient. Genetic analysis of the DNA fingerprint bands revealed no linkage or allelic relationship among the bands analyzed, indicating that these loci are not clustered in the Carica genome.
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References
Dallas, J.F., 1988. Detection of DNA ‘fingerprints’ of cultivated rice by hybridization with a human minisatellite DNA probe. Proc. Natl. Acad. Sci. USA 85: 6831–6835.
Dunnington, E.A., O. Gal, P.R. Siegal, A. Haberfeld, A. Cahaner, Y. Plotsky, U. Lavi & J. Hillel, 1991. Deoxyribonucleic acid fingerprint comparisons between selected populations of chickens. Poultry Sci. 70: 463–467.
Feinberg, A.P. & B. Vogelstein, 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Ann. Biochem. 132: 6–13.
Fritsch, E.F., J. Sambrook & T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual, 2nd edition. Cold Spring Harbor University Press, Cold Spring Harbor.
Haberfeld, A., A. Cahaner, O. Yoffe, Y. Plotsky & J. Hillel, 1991. DNA fingerprint of farm animals generated by micro and minisatellites DNA probes. Anim. Genet. 22: 299–305.
Hillel, J., Y. Plotsky, A. Haberfeld, U. Lavi, A. Cahaner & A.J. Jeffreys, 1989. DNA fingerprints of poultry. Anim. Gen. 10: 145–155.
Hillel, J., T. Schaap, A. Haberfeld, A.J. Jeffreys, Y. Plotsky, A. Cahaner & U. Lavi, 1990. Genomic selection application of DNA fingerprint for efficient gene introgression. Genetics 124: 783–789.
Jeffreys, A.J., V. Wilson & S.L. Thein, 1985a. Hypervariable ‘minisatellite’ regions in human DNA. Nature 314: 67–73.
Jeffreys, A.J., V. Wilson & S.L. Thein, 1985b. Individual-specific ‘fingerprints’ of human DNA. Nature 316: 76–79.
Jeffreys, A.J., V. Wilson, S.L. Thein, D.J. Weatherall & B.A.J. Ponder, 1986. DNA fingerprints and segregation analysis of multiple markers in human pedigrees. Am. J. Hum. Genet. 39: 11–24.
Jeffreys, A.J. & D.M. Morton, 1987. DNA fingerprints of dogs and cats. Anim. Genet. 18: 1–15.
Kuhnlein, U., Y. Dawe, D. Zadworny & J.S. Gavora, 1989. DNA fingerprinting: A tool for determining genetic distances between strains of poultry. Theor. Appl. Genet. 77: 669–672.
Kuhnlein, U., D. Zadworny, Y. Dawe, R.W. Fairfull & J.S. Gavora, 1990. Assessment of inbreeding by DNA fingerprinting: Development of a calibration curve using defined strains of chickens. Genetics 125: 161–165.
Litz, R.E. & R.A. Conover, 1979. Development of systems for obtaining parasexual Carica hybrids. Proc. Fla. State Hort. Soc. 92: 281–283.
Manshardt, R.M. & T.F. Wenslaff, 1989a. Zygotic polyembryony in interspecific hybrids of C. papaya and C. cauliflora. J. Amer. Soc. Hort. Sci. 114 (4): 684–689.
Manshardt, R.M. & T.F. Wenslaff, 1989b. Interspecific hybridization of papaya with other Carica species. J. Amer. Soc. Hort. Sci. 114 (4): 689–694.
Murray, M.G. & W.F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 8 (13): 4321–4325.
Nurnberg, P., L. Roewer, H. Neitzel, K. Sperling, A. Popperl, J. Hundrieser, H. Poche, C. Epplen, H. Zischler & J.T. Epplen, 1989. DNA fingerprinting with the oligonucleotide probe (CAC)5: Somatic stability and germline mutation. Hum. Genet. 84: 75–78.
Nybom, H. & B.A. Schaal, 1990. DNA ‘fingerprints’ applied to paternity analysis in apples (Malus × domestica). Theor. Appl. Genet. 79: 763–768.
Paterson, A.H., J.W. DeVerna, B. Lanini & S.D. Tanksley, 1990. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato. Genetics 124: 735–742.
Plotsky, Y., A. Cahaner, A. Haberfeld, U. Lavi & J. Hillel, 1990. Analysis of genetic association between DNA fingerprint bands and quantitative traits by DNA mixes. Proceedings of the 4th World Congress on genetics applied to livestock production 13: 133–136, July 1990. Edinburgh.
Sawant, A.C., 1958. Crossing relationships in the genus Carica. Evolution 12: 263–266.
Storey, W.B., 1969. Papaya in: Outlines of perennial crop breeding in the tropics. p. 389–408. H. Veenman & Zonen N.V. Wageningen.
Vassart, G., M. Georges, R. Monsieur, H. Brocas, A.S. Lequarre & D. Cristophe, 1987. A sequence in M13 phage detects hyper-variable minisatellites in human and animal DNA. Science 235: 683–684.
Weising, K., F. Weigand, A.J. Driesel, G. Kahl, H. Zischler & J.T. Epplen, 1989. Polymorphic simple GATA/GACA repeats in plant genomes. Nucl. Acids Res. 17 (23): 10128.
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Sharon, D., Hillel, J., Vainstein, A. et al. Application of DNA fingerprints for identification and genetic analysis of Carica papaya and other Carica species. Euphytica 62, 119–126 (1992). https://doi.org/10.1007/BF00037937
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DOI: https://doi.org/10.1007/BF00037937