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Mammalian cell retention devices for stirred perfusion bioreactors

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Abstract

Within the spectrum of current applications for cell culture technologies, efficient large-scale mammalian cell production processes are typically carried out in stirred fed-batch or perfusion bioreactors. The specific aspects of each individual process that can be considered when determining the method of choice are presented. A major challenge for perfusion reactor design and operation is the reliability of the cell retention device. Current retention systems include cross-flow membrane filters, spin-filters, inclined settlers, continuous centrifuges and ultrasonic separators. The relative merits and limitations of these technologies for cell retention and their suitability for large-scale perfusion are discussed.

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Authors and Affiliations

  1. Biotechnology Laboratory and Department of Chemical and Bio- Resource Engineering, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada

    Steven M. Woodside, Bruce D. Bowen & James M. Piret

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  1. Steven M. Woodside
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  2. Bruce D. Bowen
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  3. James M. Piret
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Correspondence to James M. Piret.

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Woodside, S.M., Bowen, B.D. & Piret, J.M. Mammalian cell retention devices for stirred perfusion bioreactors. Cytotechnology 28, 163–175 (1998). https://doi.org/10.1023/A:1008050202561

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