Abstract
In situ techniques for the detection of specific chromosomes using centromeric probes and the decoration of entire chromosomes using chromosome painting are well established. However, in the deciphering of complex chromosomal aberrations it is valuable to be able to detect the centromere and the entire DNA of a specific chromosome in different colours simultaneously on the same metaphase. In this report we describe a combination of the primedin situ labelling (PRINS) technique and chromosome painting for simultaneous visualization of centromere-specific oligonucleotides and chromosome painting libraries. A key feature is that the denaturation step in the PRINS reaction is sufficient to keep the chromosomes denatured for chromosome painting. This means that PRINS and consecutive chromosome painting can be performed as a single procedure (PRINS-painting)
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Hindkjær J, Koch J, Mogensen Jet al. (1991) PRimed IN Situ labelling of nucleic acids: a technique for gene mapping, diagnostics, and functional cytogenetics.Biotech Forum Europe 12: 752–756.
Hindkjær J. Brandt CA. Kaltoft K (1993) Aneuploid malignant T cells from a patient with Szary syndrome can be visualized by in situ hybridization.Arch Dermatol 129: 1141–1145.
Hindkjær J, Koch J. Terkelsen Cet al. (1994) Fast, sensitive multicolor detection of nucleic acids in situ by PRimed IN Situ labeling (PRINS).Cytogenet Cell Genet 66: 152–154.
Kallioniemi OP, Kallioneimi A, Chen LCet al. (1992) Many different chromosomal regions may undergo amplification during cancer initiation (abstract).Am J Hum Genet 51: A40.
Koch JE, Kølvraa S, Petersen KB, Gregersen N, Bolund L (1989) Oligonucleotide-priming methods for the chromosome-specific labeling of alpha satellite DNA in situ.Chromosoma 98: 259–265.
Pedersen S, Hindkjær J. Brandt CA, Bolund L, Kølvraa S (1994) Reverse chromosome painting. In: Choo, K, ed.Methods in Molecular Biology: In Situ Hybridization Protocols; Volume 10. New Jersey: The Humana Press, in press.
Pinkel D, Landegent J, Collinset al. (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.Proc Natl Acad Sci USA 85: 9138–9142.
Telenius H, Carter NP, Bebb CEet al. (1992) Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer.Genomics 13: 718–725.
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Hindkjær, J., Brandt, C.A., Koch, J. et al. Simultaneous detection of centromere-specific probes and chromosome painting libraries by a combination of primedin situ labelling and chromosome painting (PRINS-painting). Chromosome Res 3, 41–44 (1995). https://doi.org/10.1007/BF00711160
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DOI: https://doi.org/10.1007/BF00711160