Summary
An immunocytochemical study focused on the cellular localization of tissue kallikrein along the human and guinea-pig respiratory tracts is reported. A strong immunoreactivity for tissue kallikrein was observed in the seromucous glands of the nasal mucosa, trachea, and bronchi. In these glands, the immunostaining was restricted to the serous component of the acinus whereas mucous cells showed no staining. Since no immunoreactivity to kininogen was observed in any of the tissue constituents of the human and guinea-pig respiratory tree, transudation of the substrate from plasma was considered to be the preferred mode of delivery of the kininogen into the bronchopulmonary interstitium and lumen. Our results provide morphological evidence for the well documented presence of tissue kallikrein in bronchial lavage fluids and support the hypothesis that kinins may be one of the more important mediators involved during acute episodes of asthma and rhinitis.
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Bagshaw,A. F.,Bhoola,K. D.,Lemon,M. J. C. &Whicher,J. T. (1984) Development and characterization of a radio-immunoassay to measure human tissue kallikrein in biological fluids.J. Endocrinol. 101, 173–179.
Baumgarten,C. R.,Togias,A. G.,Naclerio,R. M.,Lichtenstein,L. M.,Norman,P. S. &Proud,D. (1985) Influx of kininogens into nasal secretions after antigen challenge of allergic individuals.J. Clin. Invest. 76, 191–197.
Baumgarten,C. R.,Nichols.,R. C.,Naclerio,R. M. &Proud,D. (1986) Concentrations of glandular kallikrein in human nasal secretion increase during experimentally induced allergic rhinitis.J. Immunol. 137, 1323–1328.
Bhoola,K. D.,Bewley,J.,Crothers,D. M.,Cingi,M. I. &Figueroa,C. D. (1989) Kinin receptors on epithelial cells and smooth muscle of the trachea.Adv. Exp. Med. Biol. 247A, 421–427.
Bhoola,K. D.,Figueroa,C. D. &Worthy,K. (1992) Bioregulation of kinins: kallikreins, kininogens and kininases.Pharmacol. Rev,44, 1–80.
Christiansen,S. C.,Proud,D. K. &Cochrane,C. G. (1987) Detection of tissue kallikrein in the bronchoalveolar lavage fluids of asthmatic subjects.J. Clin. Invest. 79, 188–197.
Christiansen,S. C.,Proud,D.,Sarnoff,R. B.,Juergens,U.,Cochrane,C. G. &Zuraw,B. L. (1992) Elevation of tissue kallikrein and kinin in the airways of asthmatic subjects after endobronchial allergen challenge.Am. Rev. Resp. Dis. 145, 900–905.
Figueroa,C. D.,Caorsi,I.,Subiabre,J. &Vio,C. P. (1984) Immunoreactive kallikrein localisation in the rat kidney: an immunoelectron microscopic study.J. Histochem. Cytochem. 32, 117–121.
Figueroa,C. D.,Maciver,A. G.,Mackenzie,J. C. &Bhoola,K. D. (1988) Localisation of immunoreactive kininogen and tissue kallikrein in the human nephron.Histochemistry 89, 437–442.
Figueroa,C. D.,Gonzalez,C. B.,Müller-Esterl,W. &Bhoola,K. D. (1992) Cellular localisation of human kininogens.Agents & Actions Supplements: Recent Progress on Kinins 38/1, 617–626.
Herxheimer,H. &Stesemann,E. (1961) The effect of bradykinin aerosol in guinea-pigs and in man.J. Physiol. (Lond) 158, 38P-39P.
Hojima,Y.,Cochrane,C. G.,Wiggins,R. C.,Austen,K. F. &Stevens,R. L. (1984) In vitro activation of the contact (Hageman factor) system of plasma by heparin and chondroitin sulfate E.Blood 63, 1453–1459.
Meier, H. L., Newball, H. H., Berninger, R. W., Talamo, R. C. &Lichtenstein, L. M. (1979) Purification of lung kallikrein of anaphylaxis.J. Allergy Clin. Immunol. 63, 191-Abstract.
Proud,D. &Kaplan,A. P. (1988) Kinin formation: mechanisms and role in inflammatory disorders.Ann. Rev. Immunol. 6, 49–83.
Proud,D. &Vio,C. P. (1993) Localisation of immunoreactive tissue kallikrein in human trachea.Am. J. Respir. Cell. Mol. Biol. 8, 16–19.
Proud,D.,Togias,A.,Naclerio,R. M.,Crush,S. B.,Norman,P. S. &Lichtenstein,L. M. (1983) Kinins are generatedin vivo following nasal airway challenge of allergic individuals with allergen.J. Clin. Invest. 72, 1678–1685.
Proud,D.,Macglashan,D. W.Jr,Newball,H. H.,Schulman,E. S. &Lichtenstein,L. M. (1985) Immunoglobulin E-mediated release of a kininogenase from purified human lung mast cells.Am. Respir. Dis,132, 405–408.
Proud, D., Siekerski, E. S. &Bailey, G. S. (1987a) Human lung mast cell tryptase is a kininogenase.Fed. Proc. 46, 932-Abstract.
Proud,D.,Baumgarten,C. R.,Naclerio,R. M. &Ward,P. E. (1987b) Kinin metabolism in nasal secretions during experimentally-induced allergic rhinitis.J. Immunol. 138, 428–434.
Proud,D.,Reynolds,J. C.,LaCapra,S.,Kagey-Sobotka,A.,Lichtenstein,L. M. &Naclerio,R. M. (1988) Nasal provocation with bradykinin induces symptoms of rhinitis and a sore throat.Am. Rev. Resp. Dis. 137, 613–616.
Schachter,M. (1980) Kallikreins (kininogenases): a group of serine proteases with bioregulatory actions.Pharmacol. Rev. 31, 1–17.
Varonier,H. S. &Panzani,R. (1968) The effect of inhalations of bradykinin on healthy and atopic (asthmatic) children.Int. Arch. Allergy Appl Immuno. 34, 293–296.
Vio,C. P.,Figueroa,C. D. &Caorsi,I. (1988) Anatomical relationship between kallikrein containing tubules and the juxtaglomerular apparatus in the human kidney.Am. J. Hypertens 1, 269–271.
Whalley,E. T.,Soloman,J. A.,Modafferi,D. &Cheronis,J. C. (1992) A dimeric bradykinin antagonist, CP0127, increases survival in rat and rabbit models of endotoxinshock.Agents and Actions Supplements, Recent Progress on Kinins 38/III, 413–420.
Wirth,K.,Hock,P. J.,Albus,U.,Alpermann,H. G.,Anagnostopoulos,H.,Henke,ST.,Breipohl,G.,Konig,W.,Knolle,B. A. &Scholkens,B. A. (1991) Hoe 140, a new potent and long acting bradykinin antagonist:in vivo studies.Br. J. Pharmacol. 102, 774–777.
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Poblete, M.T., Garces, G., Figueroa, C.D. et al. Localization of immunoreactive tissue kallikrein in the seromucous glands of the human and guinea-pig respiratory tree. Histochem J 25, 834–839 (1993). https://doi.org/10.1007/BF02388115
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DOI: https://doi.org/10.1007/BF02388115