Abstract
The role of polyphosphate in 2-deoxy-D-glucose transport was studied in yeast cells, pulse-labeled with [32P]orthophosphate, by comparing the concentrations and specific activities of polyphosphate, orthophosphate and 2-dGlc-phosphate. When 2-dGlc transport was measured under aerobic conditions, it appeared that polyphosphate replenished the orthophosphate pool, indicating that polyphosphate has, at least mainly, an indirect role in sugar phosphorylation. Also in cells with a reduced respiratory capacity, due to a treatment with antimycin A, no direct role for polyphosphate in 2-dGlc transport could be detected. Under these conditions, only a very limited breakdown of polyphosphate occurred, probably because of the small decrease in the orthophosphate concentration.
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Abbreviations
- 2-dGlc:
-
2-deoxy-D-glucose
- Pi :
-
orthophosphate
- Pn :
-
polyphosphate
- SP:
-
sugar phosphate
References
Bisson LF & Fraenkel DG (1983) Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 80: 1730–1734
Bisson LF & Fraenkel DG (1984) Expression of kinase-dependent glucose uptake by Saccharomyces cerevisiae. J. Bacteriol. 159: 1013–1017
Borst Pauwels GWFH, Loef HW & Havinga E (1962) The uptake of radioactive phosphate by yeast. II. The primary phosphorylation products. Biochim. Biophys. Acta 65: 407–411
Clark JE & Wood HG (1987) Preparation of standards and determination of sizes of long-chain polyphosphates by gel electrophoresis. Anal. Biochem. 161: 280–291
Felter S & Stahl AJC (1973) Enzymes du metabolisme des polyphosphates dans le levure. III. Purification et propriétés de la polyphosphate-ADP-phosphotransferase. Biochimie 55: 245–251
Franzusoff A & Cirillo VP (1982) Uptake and phosphorylation of 2-deoxy-D-glucose by wild-type and single-kinase strains of Saccharomyces cerevisiae. Biochim. Biophys. Acta 688: 295–304
Jaspers HTA & VanSteveninck J (1976) Transport of 2-deoxy-D-galactose in Saccharomyces fragilis. Biochim. Biophys. Acta 443: 243–253
Heredia CF, De la Fuente G & Sols A (1964) Metabolic studies with 2-deoxyhexoses. I. Mechanisms of inhibition of growth and fermentation in baker's yeast. Biochim. Biophys. Acta 86: 216–223
Heredia MF & Heredia CF (1988) Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency. J. Bacteriol. 170: 2870–2872
Kulaev IS & Vagabov VM (1983) Polyphosphate metabolism in micro-organisms. In: RoseAH, MorrisJG & TempestDW (Eds) Advances in Microbial Physiology, Vol. 24 (pp 83–171) Academic Press, New York
Lang JM & Cirillo VP (1987) Glucose transport in a kinaseless Saccharomyces cerevisiae mutant. J. Bacteriol. 169: 2932–2937
McNaughton DR, Klasen GR, Loewen PC & LéJohn HB (1978) Recharacterization of fungal dinucleoside polyphosphate (HS3). Can. J. Biochem. 56: 217–226
Meredith SA & Romano AH (1977) Uptake and phosphorylation of 2-deoxy-D-glucose by wild type and respiration-deficient baker's yeast. Biochim. Biophys. Acta 497: 745–759
Naftalin RJ & Smith PM (1987) A model for accelerated uptake and accumulation of sugars arising from phosphorylation at the inner surface of the cell membrane. Biochim. Biophys. Acta 897: 93–111
Robinson NA, Goss NH & Wood HG (1984) Polyphosphate kinase from Propionibacterium shermanii: Formation of an enzymatically active insoluble complex with basic proteins and characterization of synthesized polyphosphate. Biochem. Int. 8: 757–769
Schuddemat J, Van den Broek PJA & Van Steveninck J (1986) Effect of xylose incubation on the glucose transport system of Saccharomyces cerevisiae. Biochim. Biophys. Acta 861: 489–493
Schuddemat J, Van den Broek PJA & Van Steveninck J (1988) The influence of ATP on sugar uptake mediated by the constitutive glucose carrier of Saccharomyces cerevisiae. Biochim. Biophys. Acta 937: 81–87
Schuddemat J, De BooR, Van Leeuwen CCM, Van den Broek PJA & Van Steveninck J (1989) Polyphosphate synthesis in yeast. Biochim. Biophys. Acta 1010: 191–198
Tijssen JPF, Dubbelman TMAR & Van Steveninck J (1983) Isolation and characterization of polyphosphates from the yeast cell surface. Biochim. Biophys. Acta 760: 143–148
Tijssen JPF, Van den Broek PJA & Van Steveninck J (1984) The involvement of high-energy phosphate in 2-deoxy-D-glucose transport in Kluyveromyces marxianus. Biochim. Biophys. Acta 778: 87–93
Umnov AM, Steblyak AG, Umnova NS, Masurova SÉ & Kulaev IS (1975) Possible physiological role of the system of ‘High molecular weight polyphosphates-polyphosphate phosphohydrolase’ in Neurospora crassa. Mikiobiologiya 44: 414–421
Van Steveninck J & Booij HL (1964) The role of polyphosphates in the transport mechanism of glucose in yeast cells. J. Gen. Physiol. 48: 43–60
Van Steveninck J (1966) The influence of nickelous ions on carbohydrate transport in yeast cells. Biochim. Biophys. Acta 126: 154–162
Van Steveninck J (1968) Transport and transport-associated phosphorylation of 2-deoxy-D-glucose in yeast. Biochim. Biophys. Acta 163: 386–394
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Schuddemat, J., Van Leeuwen, C.C.M., Plijter, J.J. et al. Determination of the role of polyphosphate in transport-coupled phosphorylation in the yeast Saccharomyces cerevisiae . Antonie van Leeuwenhoek 57, 159–164 (1990). https://doi.org/10.1007/BF00403950
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DOI: https://doi.org/10.1007/BF00403950