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Staining yeast cells for electron microscopy by growth in copper-containing nutrient broth

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Abstract

Copper attaches to the nucleoli and the chromosomes of yeast cells. Lindegren and Zink (1969) showed that lipolated mitochondria contain fat and inferred that fat was an energy source. The present study shows that fat is present in the nucleolus and fat is inferred to be an energy source for nucleolar metabolism. The extrusion of nucleoprotein from the nucleolus into the cytoplasm is demonstrated for the first time by electron microscopy. The copper-stained chromosomes are about 90 å in diameter and tightly packed in the nuclear vacuole.

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Authors and Affiliations

  1. Department of Microbiology, Southern Illinois University, 62901, Carbondale, Illinois, USA

    C. C. Lindegren, Paraskevi M. BeMiller, Kuo -Chun Liu & Gertrude Lindegren

Authors
  1. C. C. Lindegren
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  2. Paraskevi M. BeMiller
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  3. Kuo -Chun Liu
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  4. Gertrude Lindegren
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Additional information

This investigation was supported by PHS Training Grant No. 5 TOI GM-00593-10 from the national Institute of General Medical sciences.

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Lindegren, C.C., BeMiller, P.M., Liu, K.C. et al. Staining yeast cells for electron microscopy by growth in copper-containing nutrient broth. Antonie van Leeuwenhoek 38, 17–26 (1972). https://doi.org/10.1007/BF02328073

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  • DOI: https://doi.org/10.1007/BF02328073

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