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The growth promoter spermine interacts specifically with dermatan sulfate regions that are rich inl-iduronic acid and possess antiproliferative activity

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Abstract

We have investigated interactions between spermine, a member of the growth promoting polyamine family, and various glycosaminoglycans. By using gel chromatography and equilibrium dialysis experiments we found that spermine binds tol-iduronic acid-rich dermatan sulfate (K d, approximately 3.9×10−4 M) with an affinity similar to that between spermine and DNA. By digesting spermine-dermatan sulfate complexes with chondroitin ABC lyase, the formation of oligosaccharide fragments (tetra-to-decasaccharides) was demonstrated by polyacrylamide gel electrophoresis. Chondroitin sulfate, which is deficient inl-iduronic acid, generates no spermine-protected fragments. Analysis of protected dermatan sulfate oligosaccharides indicates that the majority of thel-iduronic acid residue is non-sulfated and in a periodate-resistant conformation. The oligosaccharides also possess antiproliferative activity.

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Authors and Affiliations

  1. Department of Medical and Physiological Chemistry, University of Lund, P.O. Box 94, S-221 00, Lund, Sweden

    Mattias Belting & Lars-Åke Fransson

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  1. Mattias Belting
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  2. Lars-Åke Fransson
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Belting, M., Fransson, LÅ. The growth promoter spermine interacts specifically with dermatan sulfate regions that are rich inl-iduronic acid and possess antiproliferative activity. Glycoconjugate J 10, 453–460 (1993). https://doi.org/10.1007/BF00737966

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  • DOI: https://doi.org/10.1007/BF00737966

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