Abstract
We present methods developed for the small-sizedDaphnia species,D. galeata, D. hyalina, D. cucullata, and their hybrids, which facilitate the use of DNA variation in population studies. Described are large-scale production of material from single genotypes ofDaphnia and subsequent extraction of total DNA. The average DNA yields ranged between 6 µg per gram wet weight (D. cucullata) and 12 µg per gram wet weight (D. galeata). For comparison, the large-sized speciesD. pulex was tested and yielded an average of 28 µg DNA per gram wet weight. The DNA isolated in this manner lends itself well to molecular genetic techniques suited for population studies.
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Baudouin, M. F. and P. Scoppa, 1975. The determination of nucleic acids in freshwater plankton and its ecological implications. Freshwater Biology 5:115–120.
Bamstedt, U. and H. R. Skjoldal, 1980. RNA concentration of zooplankton: relationship with size and growth. Limnol. Oceanogr. 25:304–316.
Crease, T. J., D. J. Stanton and P. D. N. Hebert, 1989. Polyphyletic origins of asexuality inDaphnia pulex. II. Mitochondrial-DNA variation. Evolution 43:1016–1026.
Davis, L. G., M. D. Dibner and J. F. Battey, 1986. Basic Methods in Molecular Biology, Elsevier, New York, 388 pp.
Densmore, L. D., J. W. Wright and W. M. Brown, 1985. Length variation and heteroplasmy are frequent in mitochondrial DNA from parthenogenetic and bisexual lizards (GenusCnemidophorus). Genetics 110:689–707.
Flössner, D., 1972. Die Tierwelt Deutschlands, 60. Teil, Gustav Fischer Verlag, Jena.
Hartl, D. L. and A. G. Clark, 1989. Principles of population genetics, second edition, Sinauer Associates, Sunderland, Massachusetts, 682 pp.
Hebert, P. D. N., 1978. The population biology ofDaphnia (Crustacea, Daphnidae). Biol. Rev. 53:387–426.
Hebert, P. D. N., 1987. Genotypic characteristics of the Cladocera. Hydrobiologia 145:183–193.
Hebert, P. D. N. and T. J. Crease, 1980. Clonal coexistence inDaphnia pulex (Leydig): Another planktonic paradox. Science 207:1363–1365.
Hebert, P. D. N. and R. D. Ward, 1972. Inheritance during parthenogenesis inDaphnia magna. Genetics 71:639–642.
Hrbáček, J., 1987. Systematics and biogeography ofDaphnia species in the northern temperate region. In: R. H. Peters and R. DeBernardi (eds.)Daphnia. Mem. Ist. Ital. Idrobiol. 45:37–76.
Innis, M. A., D. H. Gelfand, J. J. Sninsky and T. J. White, 1990. PCR Protocols: A Guide to Methods and Applications, Academic Press, San Diego, 482 pp.
Ish-Horowicz, D., S. M. Pinchin, P. Schedl, S. Artavanis-Tsakonas and M. E. Mirault, 1979. Genetic and molecular analysis of the 87A7 and 87C1 heat-inducible loci ofD. melanogaster. Cell 18:1351–1358.
Lamb, T. and J. C. Avise, 1986. Directional introgression of mitochondrial DNA in a hybrid population of tree frogs: the influence of mating behavior. Proc. Natl. Acad. Sci. USA 83:2526–2530.
Lynch, M., 1983. Ecological genetics ofDaphnia pulex. Evolution 38:186–203.
Maynard Smith, J., 1989. Evolutionary Genetics. Oxford University Press, Oxford, 325 pp.
McKee, M. J. and C. O. Knowles, 1987. Levels of protein, RNA, DNA, glycogen and lipid during growth and development ofDaphnia magna Straus (Crustacea: Cladocera). Freshwater Biology 18:341–351.
Moritz, C., T. E. Dowling and W. M. Brown, 1987. Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Ann. Rev. Ecol. Syst. 18:269–292.
Mort, M. A. and H. G. Wolf, 1986. The genetic structure of large-lakeDaphnia populations. Evolution 40:756–766.
Peters, R. H., 1987. Metabolism inDaphnia. In: R. H. Peters and R. DeBenardi (eds.),Daphnia, Mem. Ist. Ital. Idrobiol. 34:37–76.
Rand, D. M. and R. G. Harrison, 1989. Molecular population genetics of mtDNA size variation in crickets. Genetics 121:551–569.
Sambrook, J., E. F. Fritsch and T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, New York, 3 volumes.
Trentini, M., 1980. Chromosome numbers of nine species of Daphniidae (Crustacea: Cladocera). Genetica 54:221–223.
Wolf. H. G. and M. A. Mort, 1986. Inter-specific hybridization underlies phenotypic variability inDaphnia populations. Oecologia 68:507–511.
Zaffagnini, F., 1987. Reproduction inDaphnia. In: R. H. Peters and R. DeBernardi (eds.),Daphnia, Mem. Ist. Ital. Idrobiol. 45:254–284.
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Mort, M.A., Streit, B. Measuring molecular variation in zooplankton populations: DNA extraction from smallDaphnia species. Aquatic Science 54, 77–84 (1992). https://doi.org/10.1007/BF00877265
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DOI: https://doi.org/10.1007/BF00877265