Abstract
Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only inO. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect inO. danica were judged comparable to those found in animal cells whereas the resultsE. coli were not entirely equivalent. TheO. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.
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Abbreviations
- MD:
-
menadione sodium bisulphite
- BSO:
-
buthionine sulphoximine
- GSH:
-
gluthathione
- GSSG:
-
gluthathione disulphide
- NAC:
-
N-acetyl-L-cysteine
- OTC:
-
2-oxo-4-thiazolidine carboxylic acid
- DTT:
-
dithiothreitol
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Baker, H., DeAngelis, B., Frank, O. et al. Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system. Experientia 52, 597–599 (1996). https://doi.org/10.1007/BF01969736
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DOI: https://doi.org/10.1007/BF01969736