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Interaction of basic dyes with the thiamine transport system in Saccharomyces cerevisiae

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Summary

Basic dyes such as methylene blue and triphenyltetrazolium chloride were found to inhibit thiamine transport inSaccharomyces cerevisiae. Conversely, the reduction of methylene blue and triphenyltetrazolium chloride by yeast cells was inhibited by thiamine. A thiamine transport mutant ofSaccharomyces cerevisiae showed decreased utilization of these dyes. From the results, the possibility that the uptake of basic dyes may proceed via a membrane-bound thiamine-binding protein in the thiamine transport system of the yeast is discussed.

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Authors and Affiliations

  1. Department of Biochemistry, Kyoto Prefectural University of Medicine, Kamikyoku, Kyoto, Japan

    A. Iwashima, H. Nishino, K. Sempuku & H. Nishimura

Authors
  1. A. Iwashima
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  2. H. Nishino
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  3. K. Sempuku
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  4. H. Nishimura
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Additional information

This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan.

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Iwashima, A., Nishino, H., Sempuku, K. et al. Interaction of basic dyes with the thiamine transport system in Saccharomyces cerevisiae. Experientia 37, 473–474 (1981). https://doi.org/10.1007/BF01986142

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  • DOI: https://doi.org/10.1007/BF01986142

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