Summary
A new statistical test has been developed to detect selection on silent sites. This test compares the codon usage within a gene and thus does not require knowledge of which genes are under the greatest selection, that there exist common trends in codon usage across genes, or that genes have the same mutation pattern. It also controls for mutational biases that might be introduced by the adjacent bases. The test was applied to 62 mammalian sequences, the significant codon usage biases were detected in all three species examined (humans, rats, and mice). However, these biases appear not to be the consequence of selection, but of the first base pair in the codon influencing the mutation pattern at the third position.
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Eyre-Walker, A.C. An analysis of codon usage in mammals: Selection or mutation bias?. J Mol Evol 33, 442–449 (1991). https://doi.org/10.1007/BF02103136
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DOI: https://doi.org/10.1007/BF02103136