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Tolerance Mechanisms to Copper and Zinc Excess in Rhizophora mucronata Lam. Seedlings Involve Cell Wall Sequestration and Limited Translocation

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Abstract

Rhizophora mucronata is a common mangrove growing in habitats subjected to heavy metal (HM) contamination. Understanding their physiological responses to copper (Cu) and zinc (Zn) excess and underlying tolerance mechanisms is crucial to assess impacts of metal pollution on mangrove community. Seedlings were treated with Cu or Zn (0, 50 or 100 mg per plant) by means of a single addition. At day 3 and 7, Cu and Zn accumulation, photosynthetic efficiency, superoxide dismutase and peroxidase activity, non-protein thiols, reactive oxygen species and lipid peroxidation in roots and leaves were measured. R. mucronata restricted Cu and Zn translocation, thus accumulated HM mainly in roots while kept the leaves unaffected. However, high root HM did not induce oxidative stress nor anti-oxidative defense as HM were largely deposited in cell wall. We concluded that HM tolerance strategies of R. mucronata seedlings are exclusion and restriction of translocation to the vital photosynthetic tissue.

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Acknowledgements

This work was supported by Thailand Research Fund (TRF Grant No. MRG6080076). We also thank Asst. Prof. Dr. Siriporn Pradit for technical advice.

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Authors and Affiliations

  1. Plant Physiology Laboratory, Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand

    Supakson Torasa, Pimpakan Boonyarat & Pimchanok Buapet

  2. Coastal Oceanography and Climate Change Research Center, Prince of Songkla University, Songkhla, 90110, Thailand

    Supakson Torasa & Pimchanok Buapet

  3. Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla, 90112, Thailand

    Amornrat Phongdara

Authors
  1. Supakson Torasa
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  2. Pimpakan Boonyarat
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  4. Pimchanok Buapet
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Correspondence to Pimchanok Buapet.

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128_2019_2589_MOESM1_ESM.tif

Supplementary material 1—Fig. S1 Conceptual diagram showing mechanisms of heavy metal tolerance in Rhizophora mucronata (TIF 180 KB)

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Torasa, S., Boonyarat, P., Phongdara, A. et al. Tolerance Mechanisms to Copper and Zinc Excess in Rhizophora mucronata Lam. Seedlings Involve Cell Wall Sequestration and Limited Translocation. Bull Environ Contam Toxicol 102, 573–580 (2019). https://doi.org/10.1007/s00128-019-02589-y

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  • DOI: https://doi.org/10.1007/s00128-019-02589-y

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