Metastatic colorectal cancer requires a multidisciplinary and individualized approach. Herein, we reported the case of a young woman diagnosed with metastatic rectal cancer who received an individualized multimodal treatment strategy that resulted in a remarkable survival. There were several particular aspects of this case, such as the early onset of the disease, the successful use of conversion therapy, the application of liquid biopsy to guide treatment, and the specific nature of the bone metastasis. To offer more insights for navigating such challenges in patients with metastatic colorectal cancer, we have conducted a literature review to find more data related to the particularities of this case. The incidence of early onset colorectal cancer is on the rise. Data suggests that it differs from older-onset colorectal cancer in terms of its pathological, epidemiological, anatomical, metabolic, and biological characteristics. Conversion therapy and surgical intervention provide an opportunity for cure and improve outcomes in metastatic colorectal cancer. It is important to approach each case individually, as every patient with limited liver disease should be considered as a candidate for secondary resection. Moreover, liquid biopsy has an important role in the individualized management of metastatic colorectal cancer patients, as it offers additional information for treatment decisions. Full article

In contemporary academia, English-language reading assignments are prevalent, even in contexts where English is not the primary medium of instruction nor the local language. This presents a challenge for students with English as a foreign language (EFL) who seek to pursue a university education in their home country. This study investigates the presence of English literacy events in Swedish upper secondary schools outside of English classes and explores their implications for university readiness and educational equity. Using survey data from 163 EFL students and qualitative content analysis, students’ written comments revealed a notable absence of English literacy events in non-English subjects, with limited exposure to written English texts and occasional use of multimodal English activities. The findings underscore disparities in students’ opportunities to engage with academic English literacy practices, primarily influenced by individual factors. This paper delves into the interplay between individual dimensions, and institutional and social practices, in shaping student agency in English academic literacy practices. Full article

Green manure (GM)–rice–rice rotation is an important management practice for improving soil fertility and rice productivity. The microbiological mechanisms for the increase in grain yield in GM–rice–rice rotation remain unclear. The responses of soil biodiversity, bacterial and fungal communities, and their interactions in the GM–rice–rice rotation were investigated based on two long-term field experiments in Gaoqiao (GQ) and Nanxian (NX) in Hunan Province, south China. Results showed that rice yields were raised by 11.79% and 15.03% under GM in GQ and NX, respectively. GM promoted Shannon diversity and Pielou’s evenness and changed the community structures of bacteria and fungi. The co-occurrence network analysis found that the percentages of negative edges were higher in GM (40.79% and 44.32% in GQ and NX, respectively) than those in the corresponding winter fallow (34.86% and 29.13% in GQ and NX, respectively) in the combined bacterial–fungal networks, suggesting more stable microbial community under GM. Moreover, GM had higher percentages of bacterial–fungal and fungal–fungal edges than winter fallow, indicating that GM increased the interaction between bacteria and fungi and fungi play more essential roles in affecting soil processes under GM. The keystone taxa in GM were positively linked with C metabolism-related enzymes and soil multifunctionality, and were important in improving soil fertility and rice productivity. We concluded that the fungal community was more sensitive to GM application than the bacterial community and that keystone taxa had important influences on soil properties and rice productivity in the GM–double-rice cropping system, which can effectively support the sustainable development of the paddy field ecosystem in southern China. Full article

People living with HIV (PLWH) can exhibit impaired immune responses to vaccines. Accumulating evidence indicates that PLWH, particularly those receiving antiretroviral therapy, mount strong antibody responses to COVID-19 vaccines, but fewer studies have examined cellular immune responses to the vaccinations. Here, we used an activation-induced marker (AIM) assay to quantify SARS-CoV-2 spike-specific CD4+ and CD8+ T cells generated by two and three doses of COVID-19 vaccines in 50 PLWH receiving antiretroviral therapy, compared to 87 control participants without HIV. In a subset of PLWH, T-cell responses were also assessed after post-vaccine breakthrough infections and/or receipt of a fourth vaccine dose. All participants remained SARS-CoV-2 infection-naive until at least one month after their third vaccine dose. SARS-CoV-2 infection was determined by seroconversion to a Nucleocapsid (N) antigen, which occurred in 21 PLWH and 38 control participants after the third vaccine dose. Multivariable regression analyses were used to investigate the relationships between sociodemographic, health- and vaccine-related variables, vaccine-induced T-cell responses, and breakthrough infection risk. We observed that a third vaccine dose boosted spike-specific CD4+ and CD8+ T-cell frequencies significantly above those measured after the second dose (all p < 0.0001). Median T-cell frequencies did not differ between PLWH and controls after the second dose (p > 0.1), but CD8+ T-cell responses were modestly lower in PLWH after the third dose (p = 0.02), an observation that remained significant after adjusting for sociodemographic, health- and vaccine-related variables (p = 0.045). In PLWH who experienced a breakthrough infection, median T-cell frequencies increased even higher than those observed after three vaccine doses (p < 0.03), and CD8+ T-cell responses in this group remained higher even after a fourth vaccine dose (p = 0.03). In multivariable analyses, the only factor associated with an increased breakthrough infection risk was younger age, which is consistent with the rapid increase in SARS-CoV-2 seropositivity that was seen among younger adults in Canada after the initial appearance of the Omicron variant. These results indicate that PLWH receiving antiretroviral therapy mount strong T-cell responses to COVID-19 vaccines that can be enhanced by booster doses or breakthrough infection. Full article

While examining the potential synergies between green supply chain management (GSCM) and sustainable waste management (SWM), we aim to investigate the relationship between sustainable waste management and green supply chain practices to promote a circular economy as a strategy for achieving sustainability. To address a gap in our understanding, this study will examine how GSCM and SWM relate to one another and their effect on CE deployment’s long-term survival. The research utilized a cross-sectional design with a sample size of 50 respondents from manufacturing firms. The companies were selected using purposive sampling based on varying industry sizes and operations. The findings demonstrate considerable positive correlations among GSCM practices, SWM techniques, and CE adoption, making it evident that comprehensive sustainability methods are required. Stakeholder participation, circular business models, and better governmental frameworks to promote circularity are among the many recommendations. By providing empirical evidence for the connections between GSCM, SWM, and CE, this study contributes to the current body of knowledge. It emphasizes the value of all-encompassing sustainability strategies for reaping financial and environmental benefits. Full article

The study of particle deposition in bends is always a continuous challenge in various engineering and industrial applications. New types of channels with special microstructures on the surfaces can be effective in modifying the flow field structure as well as particle deposition in channels. In this study, a 90° circular bend with a convex dimple structure was used, and the flow field and the deposition of particles in the channel were analyzed; the Stokes numbers (St) used were 0.016, 0.355 and 1.397. The reliability of the model was ensured by mesh-independence validation as well as speed validation. In a 90° bend channel with convex dimples, the temperature distribution, particle deposition distribution, flow structure and secondary flow were examined. The effects of the number of convex dimples and St in the bend on the flow field structure and particle deposition characteristics were analyzed. The results show that the main factors affecting the deposition characteristics of particles in bends are St, gravitational deposition, thermophoretic force, turbulent vortex clusters and secondary flow distribution. The effect of St is more pronounced, with the deposition rate increasing as the St increases, and the deposition location of the particles is mainly clustered on the outside of the bend structure of the elbow. Full article

Group A Streptococcus (GAS) presents a significant global health burden due to its diverse clinical manifestations ranging from mild infections to life-threatening invasive diseases. While historically stable, the incidence of GAS infections declined during the COVID-19 pandemic but resurged following the relaxation of preventive measures. Despite general responsiveness to β-lactam antibiotics, there remains an urgent need for a GAS vaccine due to its substantial global disease burden, particularly in low-resource settings. Vaccine development faces numerous challenges, including the extensive strain diversity, the lack of suitable animal models for testing, potential autoimmune complications, and the need for global distribution, while addressing socioeconomic disparities in vaccine access. Several vaccine candidates are in various stages of development, offering hope for effective prevention strategies in the future. Full article

Outbreaks of the oriental fruit fly, Bactrocera dorsalis (Hendel), present significant challenges to global fruit production, necessitating effective control measures that minimize environmental risks and pesticide resistance. This study aimed to develop and evaluate the effectiveness of four distinct push–pull control strategies for managing B. dorsalis outbreaks in a Nephelium lappaceum orchard. These strategies involved the inclusion of low-concentration abamectin, spraying repellent with a drone or manually, using methyl eugenol (ME) or food bait and employing either two types of attractants and repellents or a single type. The findings indicated that incorporating the low-concentration abamectin into the push–pull system, utilizing ME as an attractant instead of food lures and manually applying abamectin and attractants were all effective in reducing the B. dorsalis population size and minimizing fruit damage. While increasing the diversity of repellents and attractants enhanced the long-term effectiveness of the system, it did not result in a significant decrease in B. dorsalis population size or fruit damage rate compared to using a single repellent or attractant. In conclusion, the push–pull strategy emerged as a viable method for managing B. dorsalis outbreaks, offering potential benefits in reducing environmental risks and pesticide resistance. However, the study underscored the importance of the context-specific construction of push–pull strategies to optimize their effectiveness in orchard settings. Full article

The negative energy balance occurring in the periparturient period of cows will impede their health and postpartum performance. To target this issue, L-tryptophan was supplied to the prepartum cows. The results showed that L-tryptophan supplementation significantly increased the serum melatonin level and was accompanied with increases in SOD activity, IL-10 and colostrum IgA levels as well as decreases in MDA and IL-6 levels compared to the control cows. The incidence of postpartum diseases was significantly lower and the pregnancy rate was significantly higher in cows fed L-tryptophan than in the control group. A striking observation was that prepartum L-tryptophan supplementation not only improved the milk production but also the quality compared to the control cows. In general, supplementation with L-tryptophan in the prepartum period can improve the postpartum reproduction and lactation performance of cows to some extent. Full article

As the energy density and efficiency requirements of air compressors continue to increase, gas foil thrust bearings face a high risk of thermal failure due to their elevated speed and limited cooling space. This paper proposes a novel structure for gas foil thrust bearings with enhanced thermal characteristics. A thermo-elastic–hydrodynamic model is developed using a thermal-fluid–solid interaction approach to investigate aerodynamic and thermal performance. The load capacity and thermal characteristics of nine different boundary angles are analyzed. The model is validated, and the actual characteristics of gas foil bearings with various boundary angles are examined using a test rig. The results indicate that, compared to conventional gas foil thrust bearings with a boundary angle of 0°, the new structure with a boundary angle ranging from −10° to −5° not only maintains the load carrying capacity but also improves thermal characteristics. Furthermore, this improvement becomes more pronounced with higher rotational speeds. Therefore, the proposed optimization is advantageous in reducing the risk of thermal failure. Full article

The profile of secondary metabolites present in the apple cuticular layer is not only characteristic of a particular apple cultivar; it also dynamically reflects various external factors in the growing environment. In this study, the possibility of authenticating apple samples by analyzing their cuticular layer extracts was investigated. Ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) was employed for obtaining metabolomic fingerprints. A total of 274 authentic apple samples from four cultivars harvested in the Czech Republic and Poland between 2020 and 2022 were analyzed. The complex data generated, processed using univariate and multivariate statistical methods, enabled the building of classification models to distinguish apple cultivars as well as their geographical origin. The models showed very good performance in discriminating Czech and Polish samples for three out of four cultivars: “Gala”, “Golden Delicious” and “Idared”. Moreover, the validity of the models was tested over several harvest seasons. In addition to metabolites of the triterpene biosynthetic pathway, the diagnostic markers were mainly wax esters. “Jonagold”, which is known to be susceptible to mutations, was the only cultivar for which an unambiguous classification of geographical origin was not possible. Full article

The continuing mutability of the SARS-CoV-2 virus can result in failures of diagnostic assays. To address this, we describe a generalizable bioinformatics-to-biology pipeline developed for the calibration and quality assurance of inactivated SARS-CoV-2 variant panels provided to Radical Acceleration of Diagnostics programs (RADx)-radical program awardees. A heuristic genetic analysis based on variant-defining mutations demonstrated the lowest genetic variance in the Nucleocapsid protein (Np)- C-terminal domain (CTD) across all SARS-CoV-2 variants. We then employed the Shannon entropy method on (Np) sequences collected from the major variants, verifying the CTD with lower entropy (less prone to mutations) than other Np regions. Polyclonal and monoclonal antibodies were raised against this target CTD antigen and used to develop an Enzyme-linked immunoassay (ELISA) test for SARS-CoV-2. Blinded Viral Quality Assurance (VQA) panels comprised of UV-inactivated SARS-CoV-2 variants (XBB.1.5, BF.7, BA.1, B.1.617.2, and WA1) and distractor respiratory viruses (CoV 229E, CoV OC43, RSV A2, RSV B, IAV H1N1, and IBV) were assembled by the RADx-rad Diagnostics core and tested using the ELISA described here. The assay tested positive for all variants with high sensitivity (limit of detection: 1.72–8.78 ng/mL) and negative for the distractor virus panel. Epitope mapping for the monoclonal antibodies identified a 20 amino acid antigenic peptide on the Np-CTD that an in-silico program also predicted for the highest antigenicity. This work provides a template for a bioinformatics pipeline to select genetic regions with a low propensity for mutation (low Shannon entropy) to develop robust ‘pan-variant’ antigen-based assays for viruses prone to high mutational rates. Full article

Goose erysipelas is a serious problem in waterfowl breeding in Poland. However, knowledge of the characteristics of Erysipelothrix rhusiopathiae strains causing this disease is limited. In this study, the antimicrobial susceptibility and serotypes of four E. rhusiopathiae strains from domestic geese were determined, and their whole-genome sequences (WGSs) were analyzed to detect resistance genes, integrative and conjugative elements (ICEs), and prophage DNA. Sequence type and the presence of resistance genes and transposons were compared with 363 publicly available E. rhusiopathiae strains, as well as 13 strains of other Erysipelothrix species. Four strains tested represented serotypes 2 and 5 and the MLST groups ST 4, 32, 242, and 243. Their assembled circular genomes ranged from 1.8 to 1.9 kb with a GC content of 36–37%; a small plasmid was detected in strain 1023. Strains 1023 and 267 were multidrug-resistant. The resistance genes detected in the genome of strain 1023 were erm47, tetM, and lsaE-lnuB-ant(6)-Ia-spw cluster, while strain 267 contained the tetM and ermB genes. Mutations in the gyrA gene were detected in both strains. The tetM gene was embedded in a Tn916-like transposon, which in strain 1023, together with the other resistance genes, was located on a large integrative and conjugative-like element of 130 kb designated as ICEEr1023. A minor integrative element of 74 kb was identified in strain 1012 (ICEEr1012). This work contributes to knowledge about the characteristics of E. rhusiopathiae bacteria and, for the first time, reveals the occurrence of erm47 and ermB resistance genes in strains of this species. Phage infection appears to be responsible for the introduction of the ermB gene into the genome of strain 267, while ICEs most likely play a key role in the spread of the other resistance genes identified in E. rhusiopathiae. Full article

In mammals, hearing loss is irreversible due to the lack of the regenerative capacity of the auditory epithelium. However, stem/progenitor cells in mammalian cochleae may be a therapeutic target for hearing regeneration. The ubiquitin proteasome system plays an important role in cochlear development and maintenance. In this study, we investigated the role of ubiquitin C-terminal hydrolase L1 (UCHL1) in the process of the transdifferentiation of auditory supporting cells (SCs) into hair cells (HCs). The expression of UCHL1 gradually decreased as HCs developed and was restricted to inner pillar cells and third-row Deiters’ cells between P2 and P7, suggesting that UCHL1-expressing cells are similar to the cells with Lgr5-positive progenitors. UCHL1 expression was decreased even under conditions in which supernumerary HCs were generated with a γ-secretase inhibitor and Wnt agonist. Moreover, the inhibition of UCHL1 by LDN-57444 led to an increase in HC numbers. Mechanistically, LDN-57444 increased mTOR complex 1 activity and allowed SCs to transdifferentiate into HCs. The suppression of UCHL1 induces the transdifferentiation of auditory SCs and progenitors into HCs by regulating the mTOR pathway. Full article

A Trombe wall is a widely applied heating system that has a single function. An interesting thing is that both the solar heat and UV light received by a Trombe wall have an air sterilization effect. Here, the air sterilization and thermal performances of a Trombe wall in different cities were investigated based on an established heat and mass transfer model. The main results were as follows: (1) UV dose accumulation and high temperature were the most important factors that affect the UV and thermal sterilization performance, respectively. The Trombe wall had the thermal characteristics of high accumulation of UV doses in the morning and afternoon and a high temperature level at noon, which was a good match with the UV and thermal sterilization process. (2) A typical sterilization process in a Trombe wall was divided into three areas: the UV inactivation area, UV and thermal inactivation area and UV inactivation area. (3) The weather conditions played an important role in the sterilization performance. UV sterilization was dominant in cloudy weather, and thermal sterilization was dominant in sunny weather. (4) In Nanjing, Shanghai, Xining and Guangzhou, the average daily clean air volumes in heating months were 39.4, 33.5, 32.2 and 39.8 m³/m², respectively. (5) When the wall height increased from 1.5 m to 3.5 m, the average daily clean air volume in heating months increased from 31.7 to 43.6 m³/m². Full article

Skewness and bimodality properties are frequently observed when analyzing environmental data such as wind speeds, precipitation levels, and ambient temperatures. As an alternative to modeling data exhibiting these properties, we propose a flexible extension of the skew-logistic distribution. The proposal corresponds to a weighted version of the skewed logistic distribution, defined by a parametric weight function that allows shapes with up to three modes for the resulting density. Parameter estimation via the maximum likelihood approach is discussed. Simulation experiments are carried out to evaluate the performance of the estimators. Applications to environmental data illustrating the utility of the proposal are presented. Full article

Plasterboard, which serves as a nonstructural building material, is widely employed for lightweight wall construction and surface finishing in walls and ceilings. Amid mounting concerns regarding product sustainability and the adoption of Net Zero strategies, evaluating the environmental performance of materials has become crucial. This study aims to conduct a comprehensive life cycle assessment (LCA) for wall gypsum plasterboard, aiming to pinpoint areas for potential environmental improvement. The LCA methodology, adhering to established guidelines and considering midpoint impact categories, was employed to quantify environmental impacts across various stages of the plasterboard life cycle—encompassing raw material extraction, plasterboard manufacturing, transportation during all stages, and end-of-life treatment of plasterboard waste. Primary data were sourced directly from a plasterboard manufacturer and recycler and supplemented with secondary data obtained from the Environmental Product Declaration (EPD) and the Ecoinvent 3.9 database. Among the identified impact categories, the human carcinogenic toxicity category emerged as the most affected category, primarily due to the raw material supply stage, followed by freshwater ecotoxicity, which was impacted due to the material supply stage. Full article

This study addresses a crucial gap in understanding the impact of urban morphologies on the canopy urban heat islands (CUHI) effect. The selection of reference stations lacks a unified standard, and their surface air temperature (SAT) sequences are also inevitably influenced by urbanization. However, synchronous observational data from relocated meteorological stations could provide high-quality sample data for studying CUHI. Utilizing remote sensing techniques, the findings of this paper revealed that the observation environment of stations after relocation exhibited remarkable representativeness, with their observation sequences accurately reflecting the local climatic background. The differences in synchronized observation sequences could characterize the CUHI intensity (CUHII). Among the various factors, land use parameters and landscape parameters played particularly significant roles. Furthermore, the fitting performance of the random forest (RF) model for both training and testing data was significantly superior to that of the linear model and support vector regression (SVR) model. Additionally, the influence of local circulation on CUHI could not be overlooked. The mechanisms by which urban morphologies affect CUHII under different circulation backgrounds deserve further investigation. Full article

A new afterburner installation is proposed, fueled with pure hydrogen (100%H₂) or hydrogen–methane mixtures (60% H₂ + 40% CH_4, 80% H₂ + 20% CH₄) for use in cogeneration applications. Two prototypes (P1 and P2) with the same expansion angle (45 degrees) were developed and tested. P1 was manufactured by the classic method and P2 by additive manufacturing. Both prototypes were manufactured from Inconel 625. During the tests, analysis of flue gas (CO₂, CO, and NO concentration), PIV measurements, and noise measurements were conducted. The flue gas analysis emphasizes that the behavior of the two tested prototypes was very similar. For all three fuels used, the CO₂ concentration levels were slightly lower in the case of the additive-manufactured prototype P2. The CO concentration levels were significantly higher in the case of the additive-manufactured prototype P2 when 60% H₂/40% CH₄ and 80% H₂/20% CH₄ mixtures were used as fuel. When pure H₂ was used as fuel, the measured data suggest that no additional CO was produced during the combustion process, and the level of CO was similar to that from the Garrett micro gas turbine in all five measuring points. The NO emissions gradually decreased as the percentage of H₂ in the fuel mixture increased. The NO concentration was significantly lower in the case of the additive-manufactured prototype (P2) in comparison with the classic manufactured prototype (P1). Examining the data obtained from the PIV measurements of the flow within the mixing region shows that the highest axial velocity component value on the centerline was measured for the P1 prototype. The acoustic measurements showed that a higher H₂ concentration led to a reduction in noise of approximately 1.5 dB for both afterburner prototypes. The outcomes reveal that the examined V-gutter flame holder prototype flow was smooth, without any perpendicular oscillations, without chaotic motions or turbulent oscillations to the flow direction, across all tested conditions, keeping constant thermal power. Full article

In ultraviolet (UV) communication, On–Off Keying (OOK) is the primary modulation technique. Compared to OOK, frequency modulation offers stronger resistance to path attenuation. Currently, research on frequency modulation demodulation schemes for UV communication is limited, mainly employing waveform detection and laser pulse response methods, which require high detection sensitivity to light. This study presents a novel frequency modulation communication scheme using discrete Poisson channel distribution and optical pulse signal processing algorithms, enhancing the signal processing sensitivity of the existing frequency modulation scheme to the level of photons. The proposed system model is rigorously evaluated through theoretical derivations and simulations. Additionally, a hardware system integrating optical pulse counting, frequency detection, and clock data recovery algorithms is developed. Experimental results show the system achieving a 5 kbps transmission rate under frequency modulation. In non-line-of-sight (NLOS) scenarios, communication reaches up to 65 m, with the receiver elevation angle ranging from 10° to 25° and the bit error rate (BER) stabilized at 10⁻⁴, while in line-of-sight (LOS) situations, the BER remains at 10⁻⁵ up to 400 m and 10⁻⁴ up to 700 m, achieving the farthest distance and fastest communication rate achievable in the current FSK modulation scheme of ultraviolet communication systems. The integrated components enhance its applicability in communication systems. This study offers a valuable addition to UV communication technology. Full article

The phenomenon known as urban heat islands (UHIs) is becoming more common and widespread, especially in large cities and metropolises around the world. The main cause of these temperature variations between the city center and the suburbs is the replacement of large tracts of natural land with artificial (built-up) surfaces that absorb solar heat and radiate it back at night. UHIs have been the subject of numerous studies, most of which were about defining the main characteristics, factors, indexes, etc., of UHIs using remote sensing technologies or about determining mitigating activities. This paper provides a comprehensive overview of the literature, as well as a bibliometric analysis, to discover research trends related to the application of decision support systems and multi-criteria decision-making for UHI management, with a special emphasis on fuzzy theory. Data collection is conducted using the Scopus bibliographic database. Throughout the literature review, it was found that there were not many studies on multi-criteria analysis and decision support system applications regarding UHIs. The fuzzy theory application was also reviewed, resulting in only a few references. However, this topic is current, with an increase in published papers, and authors see this as an opportunity for improvement and further research. Full article